Identification of Complex and Combined Antibody Consisted of Anti-c, Anti-E, Anti-Jka and Anti-Fya.
10.19746/j.cnki.issn.1009-2137.2023.05.035
- Author:
Ting-Ting MA
1
;
Xue-Jun LIU
1
;
Bao-Jia HUANG
1
;
Yan ZHOU
1
;
Qiu-Hong MO
1
;
Zhou-Lin ZHONG
1
;
Jin-Lian LIU
2
Author Information
1. Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
2. Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.E-mail: liujinlian0121@163.com.
- Publication Type:Journal Article
- Keywords:
anti-E;
anti-Fy;
anti-Jk;
anti-c;
antibody identification;
serological method
- MeSH:
Humans;
Papain;
Blood Group Antigens;
Erythrocytes;
Immunoglobulin G;
Immunoglobulin M
- From:
Journal of Experimental Hematology
2023;31(5):1475-1480
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of multiple serological methods in the identification of complex antibodies.
METHODS:The blood group antigens were detected by saline and microcolumn agglutination methods. The saline method was used to screen and identify IgM-type antibodies in the patient's serum, while the polybrene, anti-globulin, microcolumn agglutination, enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies.
RESULTS:The patient was B/CCDee/Jk(a-b+)/Fy(a-b+) blood type. The serum reacted with panel cells, and the reaction presented anti-E pattern in the saline medium. It was fully positive in the microcolumn agglutination card, except 2 negative ones after using papain to treat the panel cells. Referring to the pattern table, it was concluded that there existed anti-c, anti-E, and anti-Jka antibodies, and one antibody corresponding to an antigen that was easily destroyed by papain. The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies.
CONCLUSION:It is confirmed that IgM-type anti-E, and IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies exist in the patient's serum by multiple serological methods.
