Melatonin-Mediated Inhibitory Effect on Hyperimmune Status of Acquired Aplastic Anemia.
10.19746/j.cnki.issn.1009-2137.2023.05.033
- Author:
Meng-Ying GAO
1
;
Mei-Li GE
2
;
Jia-Li HUO
1
;
Xing-Xin LI
1
;
Ying-Qi SHAO
1
;
Jin-Bo HUANG
1
;
Xiang REN
1
;
Jing ZHANG
1
;
Min WANG
1
;
Neng NIE
1
;
Peng JIN
1
;
Yi-Zhou ZHENG
3
Author Information
1. Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Tianjin 300020, China.
2. Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Tianjin 300020, China.E-mail: gemeili503@126.com.
3. Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Tianjin 300020, China.E-mail: zheng_yizhou@hotmail.com.
- Publication Type:Journal Article
- Keywords:
T cells;
aplastic anemia;
immunoregulation;
melatonin
- MeSH:
Humans;
Anemia, Aplastic;
CD8-Positive T-Lymphocytes;
Melatonin;
Blood Cell Count
- From:
Journal of Experimental Hematology
2023;31(5):1462-1468
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the expression level of melatonin and its effects on immune function in aplastic anemia (AA) patients.
METHODS:The enzyme-linked immunosorbent assay (ELISA) was used to detect the plasma levels of melatonin in AA patients, and the correlation between melatonin levels and laboratory indexs was analyzed. The activation, proliferation, and apoptosis of T cells from AA patients were analyzed by flow cytometry with or without melatonin in vitro.
RESULTS:The plasma levels of melatonin in AA patients were significantly lower compared with healthy controls (HC) (12.23 pg/ml vs 20.04 pg/ml, P < 0.01), while the plasma melatonin levels of AA patients in remission group after immunosuppressive therapy (IST) were significantly higher than those in non-remission group (29.16 pg/ml vs 11.73 pg/ml, P =0.04). Moreover, the melatonin levels were positively correlated with platelets (r =0.49), the absolute reticulocyte count (r =0.45), and the percentage of neutrophils (r =0.43). Meanwhile, there was a negative correlation between melatonin levels and the percentages of lymphocytes (r =-0.45). The expressions of CD25 and CD69 in both CD4+ and CD8+ T cells from AA patients were remarkably inhibited by melatonin in vitro (all P < 0.05). When cultured with melatonin, the proliferation rates of both CD4+ and CD8+ T cells from AA patients were markedly suppressed (P =0.01 andP < 0.01).
CONCLUSION:The plasma levels of melatonin were decreased in AA patients, which might play an important role in the mechanism of immunological abnormalities. The hyperimmune status of AA patients could be partially ameliorated by melatonin in vitro.
