Effect of Hispidulin on Proliferation and Apoptosis of Leukemia K562 Cells by Up-Regulating IL-37.
10.19746/j.cnki.issn.1009-2137.2023.05.011
- Author:
Hao TANG
1
;
Hui SUN
1
;
Fei-Fei YANG
2
Author Information
1. Department of Pediatrics, Tengzhou Central People's Hospital, Tengzhou 277500, Shandong Province, China.
2. Department of Pediatrics, Tengzhou Central People's Hospital, Tengzhou 277500, Shandong Province, China E-mail: fig6ee@163.com.
- Publication Type:Journal Article
- Keywords:
apoptosis;
hispidulin;
interleukin-37;
leukemia;
proliferation
- MeSH:
Humans;
K562 Cells;
bcl-2-Associated X Protein/pharmacology*;
Apoptosis;
Leukemia;
Proto-Oncogene Proteins c-bcl-2;
Cell Proliferation
- From:
Journal of Experimental Hematology
2023;31(5):1322-1326
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and underlying mechanism of hispidulin on the proliferation and apoptosis of leukemia K562 cells.
METHODS:K562 cells were cultured in vitro and treated with 0, 5, 25 or 100 μmol/L hispidulin for 24 h. Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry, respectively. Western blot was used to assess the expression of Bax, Bcl-2 and interleukin (IL)-37 proteins. Bone marrow mononuclear cells were extracted from 17 chronic myeloid leukemia patients and 21 healthy individuals by Ficoll-Hypaque density gradient method, and the expression of IL-37 protein was measured by Western blot. K562 cells with IL-37 overexpression or knockdown were constructed, and then treated with 0 or 100 μmol/L hispidulin for 24 h. Cell proliferation, apoptosis and protein expression of Bax and Bcl-2 were determined in the same way as above.
RESULTS:After K562 cells were treated with hispidulin, the cell inhibition rate, apoptosis rate, and the protein expression of Bax and IL-37 were significantly increased (P <0.05), but the cell proliferation and expression of Bcl-2 protein were decreased (P <0.05). The expression of IL-37 protein in bone marrow mononuclear cells of the leukemia patient was 0.24±0.03, which was significantly lower than 0.91±0.05 of healthy controls (P <0.05). Overexpression of IL-37 significantly promoted inhibition rate, apoptosis rate, and expression of Bax protein in K562 cells (P <0.05), but suppressed the expression of Bcl-2 protein (P <0.05). In addition, knockdown of IL-37 could reverse the effects of hispidulin on proliferation and apoptosis of K562 cells.
CONCLUSION:Hispidulin inhibits the proliferation and induces apoptosis of leukemia K562 cells, which may be related to the up-regulation of IL-37 protein in cells.
