Effects of Methionine Restriction on Proliferation, Cell Cycle, and Apoptosis of Human Acute Leukemia Cells.
10.19746/j.cnki.issn.1009-2137.2023.05.006
- Author:
Yu-Jie HE
1
;
Si-Shu YU
1
;
Bin ZHANG
1
;
Mei-Rong LI
1
;
Li-Jun XU
1
;
Long-Ming LIANG
1
;
Zheng-Gang ZHAO
1
;
Zi-Jian ZHAO
1
;
Su-Jin ZHOU
2
;
Fang-Hong LI
3
Author Information
1. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, Guangdong Province, China.
2. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, Guangdong Province, China.E-mail: zhousj@gdut.edu.cn.
3. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, Guangdong Province, China.E-mail: fli@gdut.edu.cn.
- Publication Type:Journal Article
- Keywords:
acute leukemia;
apoptosis;
cell cycle;
homocysteine;
methionine;
proliferation
- MeSH:
Humans;
Cyclin B1/pharmacology*;
Cell Proliferation;
Methionine/pharmacology*;
Cell Cycle;
Apoptosis;
Leukemia, Myeloid, Acute;
Cell Division;
Cell Cycle Proteins;
Jurkat Cells;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
HL-60 Cells
- From:
Journal of Experimental Hematology
2023;31(5):1290-1295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of methionine restriction on proliferation, cell cycle and apoptosis of human acute leukemia cells.
METHODS:Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of methionine restriction on HL-60 and Jurkat cells proliferation. The effect of methionine restriction on cell cycle of HL-60 and Jurkat cells was examined by PI staining. Annexin V-FITC / PI double staining was applied to detect apoptosis of HL-60 and Jurkat cells following methionine restriction. The expression of cell cycle-related proteins cyclin B1, CDC2 and apoptosis-related protein Bcl-2 was evaluated by Western blot assay.
RESULTS:Methionine restriction significantly inhibited the proliferation of HL-60 and Jurkat cells in a time-dependent manner (HL-60: r =0.7773, Jurkat: r =0.8725), arrested the cells at G2/M phase (P < 0.001), and significantly induced apoptosis of HL-60 and Jurkat cells (HL-60: P < 0.001; Jurkat: P < 0.05). Furthermore, Western blot analysis demonstrated that methionine restriction significantly reduced the proteins expression of Cyclin B1 (P < 0.05), CDC2 (P < 0.01) and Bcl-2 (P < 0.001) in HL-60 and Jurkat cells.
CONCLUSION:Acute leukemia cells HL-60 and Jurkat exhibit methionine dependence. Methionine restriction can significantly inhibit the proliferation, promote cell cycle arrest and induce apoptosis of HL-60 and Jurkat cells, which suggests that methionine restriction may be a potential therapeutic strategy for acute leukemia.
