Effect of LncRNA GATA3-AS1 Targeting MiR-515-5p on Cell Proliferation and Apoptosis in Childhood Acute Lymphoblastic Leukemia Cells.
10.19746/j.cnki.issn.1009-2137.2023.04.016
- Author:
Yan QIN
1
;
Xue-Xue YUN
2
;
Zhong-Mei ZHENG
1
;
Qian XU
3
;
Li-Min ZUO
4
Author Information
1. Department of Emergency, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.
2. Department of Clinical Laboratory, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.
3. Department of Pediatric Hematology and Oncology, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.
4. Department of Emergency, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.E-mail: 618782186@qq.com.
- Publication Type:Journal Article
- Keywords:
cell proliferation;
apoptosis;
childhood acute lymphoblastic leukemia
- MeSH:
Child;
Humans;
MicroRNAs/metabolism*;
RNA, Long Noncoding/metabolism*;
Antagomirs/pharmacology*;
Cell Line, Tumor;
Cell Proliferation;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics*;
Apoptosis;
Gene Expression Regulation, Neoplastic;
GATA3 Transcription Factor/metabolism*
- From:
Journal of Experimental Hematology
2023;31(4):1032-1037
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of long non-coding RNA (lncRNA) GATA3 antisense RNA 1 (GATA3-AS1) targeting miR-515-5p on the proliferation and apoptosis of childhood acute lymphoblastic leukemia (ALL) cells.
METHODS:RT-qPCR was used to determine the expression of GATA3-AS1 and miR-515-5p in the plasma of controls and ALL children. Human ALL cells Jurkat were divided into si-GATA3-AS1, si-NC, miR-NC, miR-515-5p, si-GATA3-AS1+anti-miR-NC and si-GATA3-AS1+anti-miR-515-5p groups. CCK-8 assay was used to detect the cell proliferation, and flow cytometry was used to detect the cell apoptosis. The targeting relationship between GATA3-AS1 and miR-515-5p was determined by dual-luciferase reporter assay.
RESULTS:The expression level of GATA3-AS1 in the plasma of ALL children was significantly higher than that of controls (P <0.001), while the expression level of miR-515-5p was significantly lower than that of controls (P <0.001). Compared with the si-NC group, the cell inhibition rate, apoptosis rate, and miR-515-5p expression level in si-GATA3-AS1 group were significantly increased (P <0.001). Compared with the miR-NC group, the cell inhibition rate and apoptosis rate in miR-515-5p group were significantly increased (P <0.001). GATA3-AS1 could directly and specifically bind to miR-515-5p. Compared with the si-GATA3-AS1+anti-miR-NC group, the cell inhibition rate and apoptosis rate in si-GATA3-AS1+anti-miR-515-5p group were significantly decreased (P <0.001).
CONCLUSION:Down-regulation of GATA3-AS1 can inhibit proliferation and induce apoptosis of childhood ALL cells by targeting up-regulation of miR-515-5p expression.
