Leukemia Genotype Analysis of Children with Acute Lymphoblastic Leukemia in Yunnan Area.
10.19746/j.cnki.issn.1009-2137.2023.04.008
- Author:
Shu-Lan SHI
1
;
Li-Yue KUI
1
;
Shi-Mei XIA
1
;
Bai-Ling ZHOU
1
;
Rong-Jie LI
1
;
Hong-Chao JIANG
2
Author Information
1. Department of Clinical Laboratory, Kunming Children's Hospital (Yunnan Children's Medical Center), Kunming 650228, Yunnan Province, China.
2. Department of Clinical Laboratory, Kunming Children's Hospital (Yunnan Children's Medical Center), Kunming 650228, Yunnan Province, China.E-mail: Hongchaojiang@aliyun.com.
- Publication Type:Journal Article
- Keywords:
Yunnan area;
acute lymphoblastic leukemia;
children;
leukemia genes
- MeSH:
Child;
Humans;
Infant;
Child, Preschool;
Oncogene Proteins, Fusion/genetics*;
Fusion Proteins, bcr-abl/genetics*;
Core Binding Factor Alpha 2 Subunit/genetics*;
Retrospective Studies;
China;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy*;
Genotype
- From:
Journal of Experimental Hematology
2023;31(4):980-984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze 43 leukemia genes in children with acute lymphoblastic leukemia (ALL) in Yunnan province, and provide the basis for the diagnosis and treatment of children with ALL in this area.
METHODS:The clinical data of 428 children with newly diagnosed ALL in Yunnan area from January 2015 to December 2020 were retrospectively analyzed. Multiple nested PCR technology was used to detect 43 common leukemia genes.
RESULTS:Among the 428 children with ALL, 159 were positive for leukemia genes, with a positive rate of 37.15% (159/428), and a total of 15 leukemia genes were detected. Among the 159 leukemia gene-positive children, ETV6-RUNX1+ accounted for 25.79% (41/159), followed by E2A-PBX1+ and BCR-ABL+, accounting for 24.53% (39/159) and 23.27% (37/159) respectively. MLL+ accounted for 6.29% (10/159), WT1+ accounted for 4.40% (7/159), IKZF1 gene deletion and CRLF2+ accounted for 3.77% (6/159) respectively. The positive rate of MLL (46.15%) was the highest in ＜1-year old group, the positive rate of ETV6-RUNX1 (10.56%) was the highest in 1-10-year old group, and BCR-ABL+ rate (23.65%) was the highest in >10-year old group. The distribution of leukemia genes in different age groups was statistically significant (P <0.05).
CONCLUSION:The most common fusion gene of children with ALL in Yunnan is ETV6-RUNX1, followed by E2A-PBX1 and BCR-ABL.
