Clinical prospects and research strategies of long non-coding RNA encoding micropeptides.
10.3724/zdxbyxb-2023-0128
- Author:
Xinyi WANG
1
;
Zhen ZHANG
2
;
Chengyu SHI
2
;
Ying WANG
2
;
Tianhua ZHOU
3
;
Aifu LIN
4
Author Information
1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China. 22207016@zju.edu.cn.
2. College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
3. Zhejiang University Cancer Center, Hangzhou 310058, China. tzhou@zju.edu.cn.
4. College of Life Sciences, Zhejiang University, Hangzhou 310058, China. linaifu@zju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Long non-coding RNA;
Mass spectrometry;
Micropeptide;
Review;
Ribosome profiling;
Short open reading frame
- MeSH:
Female;
Pregnancy;
Humans;
RNA, Long Noncoding/genetics*;
Research Design;
Antibodies;
Carcinogenesis;
Micropeptides
- From:
Journal of Zhejiang University. Medical sciences
2023;52(4):397-405
- CountryChina
- Language:English
-
Abstract:
Long non-coding RNAs (lncRNAs) which are usually thought to have no protein coding ability, are widely involved in cell proliferation, signal transduction and other biological activities. However, recent studies have suggested that short open reading frames (sORFs) of some lncRNAs can encode small functional peptides (micropeptides). These micropeptides appear to play important roles in calcium homeostasis, embryonic development and tumorigenesis, suggesting their potential as therapeutic targets and diagnostic biomarkers. Currently, bioinformatic tools as well as experimental methods such as ribosome mapping and in vitro translation are applied to predict the coding potential of lncRNAs. Furthermore, mass spectrometry, specific antibodies and epitope tags are used for validating the expression of micropeptides. Here, we review the physiological and pathological functions of recently identified micropeptides as well as research strategies for predicting the coding potential of lncRNAs to facilitate the further research of lncRNA encoded micropeptides.
