Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.
- Author:
Cong SHEN
1
;
Jun YU
2
;
Xi ZHANG
2
;
Chen-Chen LIU
2
;
Yue-Shuai GUO
2
;
Jia-Wei ZHU
1
;
Ke ZHANG
1
;
Yi YU
1
;
Ting-Ting GAO
2
;
Shen-Min YANG
1
;
Hong LI
1
;
Bo ZHENG
1
;
Xiao-Yan HUANG
2
Author Information
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords: Strawberry Notch 1 (SBNO1); noncanonical Wnt pathway; spermatogonial stem cells
- MeSH: Adult Germline Stem Cells/metabolism*; Animals; Cell Proliferation/physiology*; Gene Knockdown Techniques; Male; Mice; Proteome; Repressor Proteins/metabolism*; Testis/metabolism*; Wnt Signaling Pathway/physiology*
- From: Asian Journal of Andrology 2019;21(4):345-350
- CountryChina
- Language:English
- Abstract: While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation and differentiation, revealing SBNO1 is associated with neonatal testicular development. To explore further the location and function of SBNO1 in the testes, we performed Sbno1 gene knockdown in mice to study the effects of SBNO1 on neonatal testicular and SSC development. Our results revealed that SBNO1 is required for neonatal testicular and SSC development in mice. Particularly, in vitro Sbno1 gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway, through Jun N-terminal kinases. Our study suggests SBNO1 maintains SSCs by promoting the noncanonical Wnt pathway.
