Combination of stromal vascular fraction and <i>Ad-COMP-Ang1</i> gene therapy improves long-term therapeutic efficacy for diabetes-induced erectile dysfunction.

Guo-nan Yin; Lin Wang; Xiang-nan Lin; Lei Shi; Zhen-lin Gao; Feng-chan Han; Ping LI; Yin-chuan Jin; Jun-kyu Suh; Ji-kan Ryu; Xiong Wang; Hai-rong Jin

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Combination of stromal vascular fraction and Ad-COMP-Ang1 gene therapy improves long-term therapeutic efficacy for diabetes-induced erectile dysfunction.

Author: Guo-Nan YIN ¹ ; Lin WANG ² ; Xiang-Nan LIN ² ; Lei SHI ² ; Zhen-Lin GAO ² ; Feng-Chan HAN ³ ; Ping LI ³ ; Yin-Chuan JIN ⁴ ; Jun-Kyu SUH ¹ ; Ji-Kan RYU ¹ ; Xiong WANG ⁵ ; Hai-Rong JIN ²
Author Information

1. National Research Center for Sexual Medicine and Department of Urology, Inha University School of Medicine, Incheon 22332, Korea.
2. Department of Urology, Yantai Yuhuangding Hospital Affiliated to Medical College of Qingdao University, Yantai 264000, China.
3. Key Laboratory for Genetic Hearing Disorders in Shandong, Binzhou Medical University, Yantai 264003, China.
4. Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264000, China.
5. Reproductive Medicine Center, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.
Publication Type:Journal Article
Keywords: angiopoietin-1; erectile dysfunction; neural cell regeneration; stromal vascular fraction
MeSH: Angiopoietin-1/genetics*; Animals; Diabetes Mellitus, Experimental/metabolism*; Endothelium, Vascular/metabolism*; Erectile Dysfunction/therapy*; Genetic Therapy/methods*; Intercellular Junctions/metabolism*; Male; Mesenchymal Stem Cell Transplantation; Mice; Penile Erection/physiology*; Permeability
From: Asian Journal of Andrology 2018;20(5):465-472
CountryChina
Language:English
Abstract: Men with diabetic erectile dysfunction (ED) respond poorly to the currently available oral phosphodiesterase-5 inhibitors. Therefore, functional therapies for diabetic ED are needed. Stromal vascular fraction (SVF) and the adenovirus-mediated cartilage oligomeric matrix angiopoietin-1 (Ad-COMP-Ang1) gene are known to play critical roles in penile erection. We previously reported that SVF and Ad-COMP-Ang1 have only a short-term effect in restoring erectile function. Further improvements to ED therapy are needed for long-lasting effects. In the present study, we aimed to test if the combination of SVF and Ad-COMP-Ang1 could extend the erection effect in diabetic ED. We found that the combination therapy showed a long-term effect in restoring erectile function through enhanced penile endothelial and neural cell regeneration. Combination therapy with SVF and Ad-COMP-Ang1 notably restored cavernous endothelial cell numbers, pericyte numbers, endothelial cell-cell junctions, decreased cavernous endothelial cell permeability, and promoted neural regeneration for at least 4 weeks in diabetic mice. In summary, this is an initial description of the long-term effect of combination therapy with SVF and Ad-COMP-Ang1 in restoring erectile function through a dual effect on endothelial and neural cell regeneration. Such combination therapy may have therapeutic potential for the treatment of diabetic ED.