Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair.
- Author:
Er-Lei ZHI
1
;
Guo-Qing LIANG
2
;
Peng LI
1
;
Hui-Xing CHEN
1
;
Ru-Hui TIAN
1
;
Peng XU
3
;
Zheng LI
1
Author Information
- Publication Type:Journal Article
- Keywords: miR-192a; nonobstructive azoospermia; seminal plasma; varicocelectomy
- MeSH: Adult; Apoptosis; Asian People; Azoospermia/surgery*; Biomarkers/analysis*; Caspase 3/analysis*; Cell Proliferation; Humans; Infertility, Male/etiology*; Male; MicroRNAs/biosynthesis*; Microsurgery; Predictive Value of Tests; Semen/metabolism*; Testis/metabolism*; Treatment Outcome; Varicocele/surgery*
- From: Asian Journal of Andrology 2018;20(4):396-399
- CountryChina
- Language:English
- Abstract: This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR)-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles (Group A: 27 men with spermatozoa found in the ejaculate after surgery; Group B: 33 men without spermatozoa found in the ejaculate after surgery) and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls (P < 0.001), and there was no significant difference between Group A and the controls (P > 0.05). Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy.