miR-185-5p alleviates the inflammatory response of acute gouty arthritis by inhibiting of IL-1β.
- Author:
Nan HOU
1
;
Xianghui MA
2
;
Wei ZHOU
1
;
Min YUAN
1
;
Liming XU
1
;
Huanxia SUN
1
;
Yifan LIU
1
;
Lining LIU
1
;
Yanjun SHI
1
;
Chunxian LI
1
;
Yanfa FU
3
Author Information
1. Department of Rheumatology and Immunology, Liaocheng People's Hospital, Liaocheng 252000, China.
2. Department of Rheumatology and Immunology, Dongying People's Hospital, Dongying 257091, China.
3. Department of Rheumatology and Immunology, Liaocheng People's Hospital, Liaocheng 252000, China. *Corresponding author, E-mail: fu51556954@163.com.
- Publication Type:Journal Article
- MeSH:
Humans;
3' Untranslated Regions;
Arthritis, Gouty/genetics*;
Interleukin-1beta/genetics*;
Interleukin-8;
Luciferases;
MicroRNAs/genetics*;
Tumor Necrosis Factor-alpha
- From:
Chinese Journal of Cellular and Molecular Immunology
2024;40(1):51-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between interleukin-1β (IL-1β) and miR-185-5p in the process of joint injury in acute gouty arthritis (AGA). Methods The serum miR-185-5p levels of 89 AGA patients and 91 healthy volunteers were detected by real-time quantitative PCR. The correlation between miR-185-5p expression level and VAS score or IL-1β expression level was evaluated by Pearson correlation coefficient method. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of miR-185-5p in AGA. THP-1 cells were induced by sodium urate (MSU) to construct an in vitro acute gouty inflammatory cell model. After the expression level of miR-185-5p in THP-1 cells was upregulated or downregulated by transfection of miR-185-5p mimics or inhibitors in vitro, inflammatory cytokines of THP-1 cells, such as IL-1β, IL-8 and tumor necrosis factor α (TNF-α), were detected by ELISA. The luciferase reporter gene assay was used to determine the interaction between miR-185-5p and the 3'-UTR of IL-1β. Results Compared with the healthy control group, the expression level of serum miR-185-5p in AGA patients was significantly reduced. The level of serum miR-185-5p was negatively correlated with VAS score and IL-1β expression level. The area under the curve (AUC) was 0.905, the sensitivity was 80.17% and the specificity was 83.52%. Down-regulation of miR-185-5p significantly promoted the expression of IL-1β, IL-8 and tumor necrosis factor (TNF-α), while overexpression of miR-185-5p showed the opposite results. Luciferase reporter gene assay showed that IL-1β was the target gene of miR-185-5p, and miR-185-5p negatively regulated the expression of IL-1β. Conclusion miR-185-5p alleviates the inflammatory response in AGA by inhibiting IL-1β.
