Comparison of two luminescence detection methods for staphylococcal enterotoxin C content in simulated milk samples.
- Author:
Yuling ZHENG
1
;
Ye WANG
1
;
Qingyu LYU
2
Author Information
1. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
2. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. *Corresponding author, E-mail: lvqingyu2004@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Streptavidin;
Biotin;
Luminescence;
Milk;
Antibodies, Monoclonal;
Goats;
Immunoassay/methods*
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(12):1089-1093
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivity and accuracy of amplified luminescent proximity homogeneous assay linked immunosorbent assay (AlphaLISA) and magnetic particles-based chemiluminescence immunoassay (MP-CLIA) for detection of staphylococcal enterotoxin C (SEC) in the simulated milk samples. Methods The AlphaLISA was constructed using goat anti-SEC polyclonal antibody-coupled receptor microspheres, biotin-labeled SEC monoclonal antibody and streptavidin-coupled donor microspheres. The MP-CLIA was constructed using goat anti-SEC polyclonal antibody conjugated alkaline phosphatase, biotin-labeled anti-SEC monoclonal antibody and streptavidin conjugated magnetic beads. Results The sensitivity of AlphaLISA to detect SEC content in simulated milk samples was 4.04 ng/L, and the coefficient of variation (CV) was 1.98%~9.82%. The sensitivity of MP-CLIA was 108.19 ng/L and CV was 4.63%~20.40%. Conclusion Compared with MP-CLIA, AlphaLISA is more sensitive and accurate to detecting SEC.
