Establishment and evaluation of pre-metastatic niche mouse model labelled with luciferase in lewis lung cancer cells.
- Author:
Haixia MING
1
;
Ming SHEN
2
;
Yanwen CHEN
3
;
Yang LI
2
;
Lingling YANG
3
;
Qiankun LIANG
3
;
Zhaohua LIU
2
Author Information
1. School of Basic Medicine, Gansu University of Chinese Medicine, Basic Discipline of Integrative Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Gansu University Key Laboratory for Molecular Medicine & Chinese Medicine Prevention and Treatment of Major Diseases, Gansu Key Laboratory of TCM Prevention and Treatment of Chronic Diseases, Lanzhou 730000, China. *Corresponding author, E-mail: 18909429885@163.com.
2. School of Basic Medicine, Gansu University of Chinese Medicine, Basic Discipline of Integrative Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Gansu University Key Laboratory for Molecular Medicine & Chinese Medicine Prevention and Treatment of Major Diseases, Gansu Key Laboratory of TCM Prevention and Treatment of Chronic Diseases, Lanzhou 730000, China.
3. School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China.
- Publication Type:Journal Article
- MeSH:
Mice;
Animals;
Lung Neoplasms/pathology*;
Matrix Metalloproteinase 9;
Mice, Inbred C57BL;
Carcinoma, Lewis Lung;
Disease Models, Animal;
RNA, Messenger;
Tumor Microenvironment
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(12):1083-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to establish a pre-metastatic niche mouse model utilizing luciferase-labeled Lewis (Luc-Lewis) lung cancer cells and to assess the efficacy of this model employing both qualitative and quantitative methods. Methods C57BL/6 mice were categorized into two groups: a normal control group and a model group, each containing 15 individual mice. The pre-metastatic niche model was established via tail vein injection of Luc-Lewis lung cancer cells. Body mass were measured daily for all groups. Tumor fluorescence signals within the mice were detected using a high-throughput enzyme marker instrument. Lung tissue specimens were harvested to evaluate metastatic progression. HE staining was used to assess histopathological changes. Real-time quantitative PCR and Western blot analysis were used to detect the mRNA and protein expression of lysyl oxidase (LOX), matrix metalloproteinase 9 (MMP9), versican (VCAN), and fibronectin (FN), which are the specific markers for the formation of the microenvironment of lung tissues before metastasis. Results Significant declines in body mass and observable lethargy were noted in the model group when compared to the control group. Distinct fluorescence signals were observed in the lung tissue of the model group, demonstrating a positive correlation with the duration of model establishment. By day 14, elevated mRNA and protein expression levels of LOX, MMP9, VCAN, and FN were significantly evident. In addition, histopathological evaluations revealed augmented interstitial thickness, alveolar atrophy and significant inflammatory cell infiltration within the lung tissues of the model group. By the 21st day, metastatic lesions manifested in the lung tissues of the model group, suggesting an approximate pre-metastatic niche maturation timeline of 14 days. Conclusion A pre-metastatic niche mouse model for Lewis lung cancer is successfully established.
