Development of a fluorescence resonance energy transfer (FRET) immunoassay for the measurement of serum insulin.
- Author:
Jie NAN
1
;
Huan XIA
2
;
Nan ZHANG
2
;
Hongwei ZHAO
2
;
Bei XU
2
;
Na SAI
3
Author Information
1. Clinical Laboratory, Tianjin Xiqing Hospital, Tianjin 300380, China.
2. Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China.
3. Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China. *Corresponding author, E-mail: saina@tmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Fluorescence Resonance Energy Transfer;
Insulin;
Immunoassay
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(10):898-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the fluorescence resonance energy transfer (FRET) effect between dylight (DL) and AuNP (AuNP), and to construct a new fluorescence immunoassay for insulin in combination with the immunocompetition method. Methods Insulin antigen (Ag) and insulin antibody (Ab) were conjugated with DL and AuNP respectively to form DL-Ag conjugate and AUNp-AB conjugate. A novel fluorescence immunoassay for insulin was developed on the basis of FRET effect and the immune competition response between them. Then the performance of the method was evaluated and its application in actual samples was explored. Results The fluorescence immunoassay showed high sensitivity (0.015 ng/mL), short measurement time (4 min) and good specificity. It was successfully used in the measurement of serum insulin, and the recovery was between 96.9% and 121.1%. Conclusion FRET effect between AuNP and DL can be applied to develop a fluorescence immunoassay for the measurement of serum insulin.
