The application of PCR-SSP with the serology in identification and genotyping of ABO ambiguous blood group.
- Author:
Yanyan SONG
1
;
Yuxi ZHANG
1
;
Xinrui CAO
1
;
Xiaonan YU
1
;
Wei ZHENG
2
Author Information
1. Department of Blood Transfusion of General Hospital of Northern Theater Command, Shenyang 110016, China.
2. Department of Blood Transfusion of General Hospital of Northern Theater Command, Shenyang 110016, China. *Corresponding author, E-mail: zhengweidr@163.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System/genetics*;
Genotype;
Polymerase Chain Reaction;
Antibodies;
DNA Primers
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(9):824-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of blood group serology and polymerase chain reaction with sequence-specific primers (PCR-SSP) on identification and genotyping of ambiguous ABO blood group. Methods Eighty suspicious ABO blood group samples were identified by serology and polymerase chain reaction with sequence-specific primers (PCR-SSP). The final blood group type and the strategy of the transfusion of each case were determined according to the results of serology and PCR-SSP. Results 40 cases were confirmed to be subtypes, and the remaining 40 cases were normal types with weakened antigens or missing antibodies due to other reasons. The results of molecular genetic blood group typing based on PCR-SSP were 41 cases of subtypes (There were 3 discrepancies between two methods: one was Ael identified by serological methods, while its gene type was O2O2; one was common type O, while its gene type was BO1; one was type A, while its gene type was AB.) and 39 cases of normal ones. Conclusion Genotyping technology combined with serological typing has an important significance in identification of ABO blood groups.
