Salidroside improves intestinal mucosal immune status of rats under compound stress of hypoxia and training via inhibiting TLR4/NF-κB signaling pathway.
- Author:
Qin XU
1
;
Hongyan PENG
2
;
Yongmei ZHAO
3
;
Tuolihanayi TUOLIKEN
4
;
Wendong BAI
5
Author Information
1. Key Laboratory of Special Environmental Medicine, General Hospital of Xinjiang Military Command, Urumqi 830000, China.
2. Department of Internal Medicine, Hospital of 63650 PLA Army, Urumqi 841700, China.
3. Department of Hematology, General Hospital of Xinjiang Military Command Urumqi 830000, China.
4. Xinjiang Agricultural University, Urumqi 830000, China.
5. Department of Hematology, General Hospital of Xinjiang Military Command Urumqi 830000, China. *Corresponding author, E-mail: bwddcgzl@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Rats;
Rats, Sprague-Dawley;
NF-kappa B;
Toll-Like Receptor 4/genetics*;
Claudin-3;
Hypoxia;
Immunoglobulin A, Secretory;
Signal Transduction
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(9):801-806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of salidroside on intestinal mucosal immune status in rats under compound stress of hypoxia and training (HTCS) and the mechanism. Methods SD rats were randomly divided into HTCS model group (model), placebo group (placebo) and salidroside group (salidro). Model group received no intervention, and placebo and salidro group received intraperitoneal injection of normal saline and salidroside, respectively. Then, ileum tissue of rats were collected and the intestinal damage was assayed by HE staining and Chiu scores. Intestinal permeability indices, including serum D-diamine oxidase (DAO), D-lactic acid (DLA) and endotoxin (END) and secretory immunoglobulin A (sIgA) of intestinal tissue were detected by ELISA. T lymphocyte subsets of intestinal tissue were detected by flow cytometry. Expression of tight junction molecules, including ZO-1, Claudin-3, occluding, were detected by PCR and western blot. Activation of TLR4/NF-κB signaling pathway was detected by Western blot analysis. Results Compared with model group and placebo group, salidro group had the decreased intestinal mucosal injury and low Chiu score, and the level of intestinal permeability indices including serum DAO, DLA and END fell off. CD4+ T cell percentage, CD4+/CD8+ ratio and sIgA level were went up, while CD8+ T cell percentage was went down. mRNA and the level of protein expressions of ZO-1, claudin-3 and occludin increased, while activation of TLR4/NF-κB signaling pathway was inhibited. Conclusion Salidroside can alleviate the intestinal barrier injury and improve intestinal mucosal immune status of rats under compound stress of hypoxia and training via inhibiting TLR4/NF-κB signalling pathway.
