Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34).
10.12116/j.issn.1004-5619.2022.520902
- Author:
Lin-Lin GAO
1
;
Wei XIE
2
;
Su-Juan ZHU
3
;
Da LI
4
;
Qin WANG
1
;
Liang HONG
1
;
You-Ying LI
1
Author Information
1. Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310006, China.
2. Forensic Judicial Appraisal Center of Xiaoshan Branch of Hangzhou Public Security Bureau, Hangzhou 311203, China.
3. Hangzhou Center for Diseases Control and Prevention, Hangzhou 310021, China.
4. Institute of Criminal Science and Technology, Taizhou Public Security Bureau, Taizhou 318000, Zhejiang Province, China.
- Publication Type:Journal Article
- Keywords:
IDentifier DNA typing kit (YanHuang34);
efficiency validation;
forensic genetics;
individual identification;
kinship;
short tandem repeat (STR)
- MeSH:
Humans;
DNA Fingerprinting;
Polymerase Chain Reaction;
Microsatellite Repeats;
Paternity;
Species Specificity
- From:
Journal of Forensic Medicine
2023;39(6):579-585
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the technical performance of IDentifier DNA typing kit (YanHuang34) and evaluate its forensic application value.
METHODS:Following the Criterion of Forensic Science Human Fluorescence STR Multiplex Amplification Reagent (GB/T 37226-2018), IDentifier DNA typing kit (YanHuang34) was verified in 11 aspects of species specificity, veracity, sensibility, adaptability, inhibitor tolerance, consistency, balance, reaction condition verification, mixed samples, stability and inter batch consistency. The system efficiency of IDentifier DNA typing kit (YanHuang34) was compared with the PowerPlex® Fusion 6C System, VersaPlex® 27PY System and VeriFilerTM Plus PCR Amplification Kit. The IDentifier DNA typing kit (YanHuang34) was used to detect the swabs of biological samples in daily cases and the STR performances were observed.
RESULTS:IDentifier DNA typing kit (YanHuang34) had good species specificity, veracity, adaptability, inhibitor tolerance and balance. The sensibility was up to 0.062 5 ng. It was able to detect different types of samples, degraded samples and inhibitor mixed samples. Complete DNA typing could be obtained for samples with the mixture ratio less than 4∶1. The system efficiency of IDentifier DNA typing kit (YanHuang34) was very high, with TDP up to 1-1.08×10-37, CPEtrio and CPEduo up to 1-5.47×10-14 and 1-6.43×10-9, respectively. For the touched biological samples in actual cases, the effective detection rate was 21.05%. The system efficiency of kinship, single parent and full sibling identifications was effectively improved.
CONCLUSIONS:The IDentifier DNA typing kit (YanHuang34) is adaptive to the GB/T 37226-2018 requirements. It can be used for individual identification and paternity identification, and is suitable for application in the field of forensic science.
