Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population.
10.12116/j.issn.1004-5619.2022.520405
- Author:
Zi-Wei WANG
1
;
Cheng-Tao LI
1
;
Xi-Ling LIU
2
Author Information
1. Department of Forensic Medicine, School of Basic Medicine and Biological Sciences, Suzhou Medical College of Soochow University, Suzhou 215123, China.
2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
DNA methylation;
age estimation;
epigenetics;
forensic genetic;
next-generation sequencing;
pyrosequencing
- MeSH:
Humans;
Aging/genetics*;
CpG Islands;
DNA Methylation;
East Asian People;
Forensic Genetics/methods*
- From:
Journal of Forensic Medicine
2023;39(5):441-446
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.
METHODS:According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.
RESULTS:The 6 DNA methylation sites in both detection techniques were age-related, with an R2 of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an R2 of 0.84 and MAD of 4.41 years when using NGS.
CONCLUSIONS:The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.
