Evaluation of the Usefulness of Kobias HBsAg and Anti-HBs.
- Author:
Joowon PARK
1
;
Insoo RHEEM
;
Do Young YOON
;
Jong Seok LIM
;
Jong Wan KIM
Author Information
1. Department of Laboratory Medicine, Dankook University College of Medicine, Cheonan, Korea. wan1818@hitel.net
- Publication Type:Original Article
- Keywords:
HBsAg;
Anti-HBs;
Kobias(R) HBsAg;
Kobias(R) Anti-HBs
- MeSH:
Diagnosis;
Emergencies;
Exercise Test;
Hepatitis B Surface Antigens*;
Hepatitis B virus;
Immunoenzyme Techniques;
Mass Screening;
Sensitivity and Specificity
- From:The Korean Journal of Laboratory Medicine
2003;23(6):411-414
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The analysis of serological markers for hepatitis B virus (HBV) is a useful tool for the prevention and diagnosis of HBV infection. In this work, we evaluated a newly improved domestic rapid assay, Kobias HBsAg and anti-HBs Window kits (Kobias, Korea) for the detection of HBsAg and anti-HBs in serum. METHODS: A total of 360 sera screened by enzyme immunoassay (EIA) (Enzygnost, DADE Behring, Germany) were included in this study. Each specimen was tested for HBsAg and anti-HBs by Kobias Window kits and Genedia Rapid device (Green Cross, Korea), conventional one step test kits. The results were compared with those of EIA. RESULTS: The sensitivity and specificity of Kobias HBsAg were 99.2% and 96.7%, and those of Kobias Anti-HBs were 95.8% and 96.7%, respectively. The concordance rates between EIA and Kobias HBsAg and Kobias Anti-HBs were 98.3% and 96.1%, and those between Kobias kits and Genedia kits for HBsAg and anti-HBs were 97.8% and 93.9%, respectively. CONCLUSIONS: Kobias HBsAg/Anti-HBs kits are simple, rapid, and low-cost methods for detecting HBsAg and anti-HBs. With comparable results with EIA, the Kobias HBsAg/Anti-HBs kits could be suitable for screening purposes or in emergency situations.
