Preliminary study on the regulation of acute myeloid leukemia by FLT3 gene expression.
10.3760/cma.j.cn511374-20220615-00410
- VernacularTitle:FLT3基因的表达对于急性髓系白血病的调控作用初探
- Author:
Sishi TANG
1
;
Yanhong ZHOU
;
Wenjing ZHOU
;
Nian WANG
;
Binwu YING
;
Yuanxin YE
Author Information
1. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. jennyyyx1982@126.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Apoptosis/genetics*;
Data Analysis;
Leukemia, Myeloid, Acute/genetics*;
Gene Expression;
fms-Like Tyrosine Kinase 3/genetics*
- From:
Chinese Journal of Medical Genetics
2023;40(9):1113-1117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To assess the influence of FLT3 expression on the prognosis of patients with acute myeloid leukemia (AML) by cell experiment and clinical data analysis.
METHODS:Models for FLT3 over-expression and interference-expression in AML cells were constructed. The level of BAK gene expression and its protein product was determined, along with the proliferation and apoptosis of leukemia cells. FLT3 gene expression and FLT3-ITD variant were determined among patients with newly diagnosed AML.
RESULTS:Compared with the interference-expression group, the level of BAK gene expression and its protein in FLT3 over-expression AML cells was significantly lower (P < 0.001), which also showed significantly faster proliferation (P < 0.001) and lower rate of apoptosis (P < 0.001). The expression level of FLT3 gene among patients with newly diagnosed AML was also significantly higher compared with the healthy controls (P < 0.001). The FLT3 gene expression of FLT3-ITD positive AML patients was higher than that of FLT3-WT patients (P = 0.002). Survival analysis showed that AML patients with high FLT3 expression in the medium-risk group had a lower complete remission rate and overall survival rate compared with those with a low FLT3 expression (P < 0.001).
CONCLUSION:Over-expression of FLT3 may influence the course of AML by promoting the proliferation of leukemia cells and inhibiting their apoptosis, which in turn may affect the prognosis of patients and serve as a negative prognostic factor for AML.
