Pro-oxidation status of rotator cuff tissue and expression of Beclin-1 and mTOR in patients with rotator cuff injury.
10.12200/j.issn.1003-0034.2023.12.006
- Author:
Zhi-Jun LIU
1
;
He-Wei WEI
1
;
Wei-Peng ZHENG
1
;
Lei WAN
1
;
Zhi-Hao LIAO
1
;
Sheng CHEN
1
Author Information
1. The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510240, Guangdong, China.
- Publication Type:Journal Article
- Keywords:
Beclin-1;
Reactive oxygen species;
Rotator cuff injury;
Superoxides;
Target of rapamucin proteins
- MeSH:
Female;
Humans;
Male;
Beclin-1/metabolism*;
Reactive Oxygen Species/metabolism*;
RNA, Messenger/metabolism*;
Rotator Cuff/surgery*;
Rotator Cuff Injuries/surgery*;
Superoxide Dismutase/metabolism*;
TOR Serine-Threonine Kinases/metabolism*
- From:
China Journal of Orthopaedics and Traumatology
2023;36(12):1136-1141
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore pro-oxidative state of rotator cuff tissue and expression levels of Beclin-1 and mam-malian target of rapamycin(mTOR) in patients with acute and chronic rotator cuff injury, and then analyzed relationship between rotator cuff injury and oxidative stress and autophagy.
METHODS:Forty patients with rotator cuff injury were seleceted from July 2019 to December 2020, and divided into male chronic injury group, male acute injury group, female chronic injury group, and female acute injury group, 10 patients in each group. All patients were performed rotator cuff repair under arthroscopy. The sample of tendon at the rotator cuff injury site of the patient was taken during operation, and total reactive oxygen species (ROS) and superoxide dismutase(SOD) were detected by detection kit;expression of Beclin-1 and mTOR mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR), and Western-blot was applied to detect protein expression of Beclin-1 and p-mTOR/mTOR.
RESULTS:There were no significant difference in expression of ROS, SOD, Beclin-1mRNA and mTOR mRNA between male and female chronic injury groups, and between male and female acute injury groups (P>0.05); ROS, SOD and Beclin-1mRNA in male chronic injury group were higher than those in male chronic injury group, while mTOR mRNAand protein decreased (P<0.05);ROS, SOD and Beclin-1 mRNA in female chronic injury group were up-regulated compared with female acute injury group, while mTOR mRNA was down-regulated (P<0.05).
CONCLUSION:Chronic rotator cuff injury is more likely to stimulate the pro-oxidation state of rotator cuff tissue than acute rotator cuff injury, which could up-regulating expression of autophagy factor Beclin-1 and down-regulating expression of mTOR. Therefore, patients with chronic rotator cuff injury may have higher levels of oxidative stress and autophagy.
