Impact of lithocholic acid on the osteogenic and adipogenic differentiation balance of bone marrow mesenchymal stem cells.
10.7507/1002-1892.202308050
- Author:
Cui WANG
1
;
Jiao LI
2
;
Lingyun LU
3
;
Lu LIU
1
;
Xijie YU
1
Author Information
1. Laboratory of Endocrinology & Metabolism/Department of Endocrinology & Metabolism, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
2. Department of Infectious Diseases, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
3. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
- Publication Type:Journal Article
- Keywords:
Lithocholic acid;
adipogenic differentiation;
bone marrow mesenchymal stem cells;
mouse;
osteogenic differentiation;
osteoporosis
- MeSH:
Female;
Mice;
Animals;
Core Binding Factor Alpha 1 Subunit/pharmacology*;
PPAR gamma/metabolism*;
Steroid 12-alpha-Hydroxylase/metabolism*;
Mice, Inbred C57BL;
Cell Differentiation;
Osteogenesis;
Mesenchymal Stem Cells;
Bile Acids and Salts/pharmacology*;
Bone Marrow Cells;
Cells, Cultured;
Azo Compounds
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2024;38(1):82-90
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To Investigate the effects of lithocholic acid (LCA) on the balance between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
METHODS:Twelve 10-week-old SPF C57BL/6J female mice were randomly divided into an experimental group (undergoing bilateral ovariectomy) and a control group (only removing the same volume of adipose tissue around the ovaries), with 6 mice in each group. The body mass was measured every week after operation. After 4 weeks post-surgery, the weight of mouse uterus was measured, femur specimens of the mice were taken for micro-CT scanning and three-dimensional reconstruction to analyze changes in bone mass. Tibia specimens were taken for HE staining to calculate the number and area of bone marrow adipocytes in the marrow cavity area. ELISA was used to detect the expression of bone turnover markers in the serum. Liver samples were subjected to real-time fluorescence quantitative PCR (RT-qPCR) to detect the expression of key genes related to bile acid metabolism, including cyp7a1, cyp7b1, cyp8b1, and cyp27a1. BMSCs were isolated by centrifugation from 2 C57BL/6J female mice (10-week-old). The third-generation cells were exposed to 0, 1, 10, and 100 μmol/L LCA, following which cell viability was evaluated using the cell counting kit 8 assay. Subsequently, alkaline phosphatase (ALP) staining and oil red O staining were conducted after 7 days of osteogenic and adipogenic induction. RT-qPCR was employed to analyze the expressions of osteogenic-related genes, namely ALP, Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN), as well as adipogenic-related genes including Adiponectin (Adipoq), fatty acid binding protein 4 (FABP4), and peroxisome proliferator-activated receptor γ (PPARγ).
RESULTS:Compared with the control group, the body mass of the mice in the experimental group increased, the uterus atrophied, the bone mass decreased, the bone marrow fat expanded, and the bone metabolism showed a high bone turnover state. RT-qPCR showed that the expressions of cyp7a1, cyp8b1, and cyp27a1, which were related to the key enzymes of bile acid metabolism in the liver, decreased significantly ( P<0.05), while the expression of cyp7b1 had no significant difference ( P>0.05). Intervention with LCA at concentrations of 1, 10, and 100 μmol/L did not demonstrate any apparent toxic effects on BMSCs. Furthermore, LCA inhibited the expressions of osteogenic-related genes (ALP, Runx2, and OCN) in a dose-dependent manner, resulting in a reduction in ALP staining positive area. Concurrently, LCA promoted the expressions of adipogenic-related genes (Adipoq, FABP4, and PPARγ), and an increase in oil red O staining positive area.
CONCLUSION:After menopause, the metabolism of bile acids is altered, and secondary bile acid LCA interferes with the balance of osteogenic and adipogenic differentiation of BMSCs, thereby affecting bone remodelling.
