Content of bone morphogenetic protein 2 in demineralized bone matrix prepared from different long bones and study of the osteogenic properties in vitro.
10.7507/1002-1892.202303132
- Author:
Yongjie ZHAO
1
;
Gang YIN
1
;
Rui DU
1
;
Limin WANG
2
;
Mingming DENG
1
;
Guofeng GUAN
1
;
Guangchao SUN
1
;
Ying LIU
1
Author Information
1. Department of Foot and Ankle Surgery, Binzhou Medical University Hospital, Binzhou Shandong, 256603, P. R. China.
2. Beijing Weidafeng Medical Biomaterials Co., Ltd, Beijing, 102200, P. R. China.
- Publication Type:Journal Article
- Keywords:
Demineralized bone matrix;
bone morphogenetic protein 2;
cell proliferation;
osteogenic differentiation
- MeSH:
Alkaline Phosphatase;
Bone Matrix;
Bone Morphogenetic Protein 2;
Cell Count;
Coloring Agents;
Osteogenesis;
Animals;
Mice
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2023;37(8):945-951
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To measure the concentration of bone morphogenetic protein 2 (BMP-2) in demineralized bone matrix (DBM) prepared from different long bones and to evaluate the osteoinductivity of different DBM on MC3T3-E1 cells.
METHODS:Different bones from the same cadaver donor were used as the initial materials for making DBM, which were divided into ulna group (uDBM), humerus group (hDBM), tibia group (tDBM), and femur group (fDBM) according to the origins, and boiled DBM (cDBM) was taken as the control group. The proteins of DBM were extracted by guanidine hydrochloride, and the concentrations of BMP-2 were determined by ELISA assay. Then the DBM were co-cultured with MC3T3-E1 cells, the proliferation of MC3T3-E1 cells was observed by cell counting kit 8 (CCK-8) assay. The osteogenic differentiation ability of MC3T3-E1 cells was qualitatively observed by alizarin red, alkaline phosphatase (ALP), and Van Gieson staining, and the osteogenic differentiation ability of MC3T3-E1 cells was quantitatively analyzed by ALP content. Linear regression was used to analyze the effect of BMP-2 concentration in DBM on ALP synthesis.
RESULTS:There were significant differences in the concentration of BMP-2 among the DBM groups (P<0.05). The concentrations of BMP-2 in the lower limb long bone were higher than those in the upper limb long bone, and the concentration of BMP-2 in the fDBM group was about 35.5 times that in the uDBM group. CCK-8 assay showed that the cells in each group continued to proliferate within 5 days of co-culture, and the absorbance (A) values at different time points were in the order of cDBM groupP<0.05). Linear regression analysis showed that y^=0.361+0.017x, the effect of BMP-2 concentration in DBM on cellular ALP content was significant (t=3.552, P=0.005); for every 1 ng/g increase in BMP-2 concentration, ALP content would increase by 0.017 [95%CI (0.006, 0.027)] U/100 mL.
CONCLUSION:The concentration of natural BMP-2 in different long bones varies greatly, and the lower limb long bone is higher than the upper limb long bone. The harvested location of bone material was an important factor affecting the osteoinductivity of DBM.
