Effects of gangliosides from deer bone extract on the gene expressions of matrix metalloproteinases and collagen type II in interleukin-1β-induced osteoarthritic chondrocytes.
10.4162/nrp.2016.10.6.569
- Author:
Hyung Joo SUH
1
;
Hyunji LEE
;
Byung Jung MIN
;
Sung Ug JUNG
;
Eun Young JUNG
Author Information
1. Department of Public Health Sciences, Korea University, Seoul 02841, Korea.
- Publication Type:Original Article
- Keywords:
Chondrocytes;
collagen type II;
interleukin-1β;
matrix metalloproteinases;
osteoarthritis
- MeSH:
Cell Death;
Cell Survival;
Chondrocytes*;
Chondroitin;
Collagen Type II*;
Collagen*;
Deer*;
Down-Regulation;
Gangliosides*;
Gene Expression*;
Glucosamine;
Matrix Metalloproteinases*;
Osteoarthritis;
RNA, Messenger
- From:Nutrition Research and Practice
2016;10(6):569-574
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-1β-induced osteoarthritis (OA) chondrocytes. MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-1β treatment), PC (IL-1β + 100 µg/mL glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-1β + 100 µg/mL deer bone extract). RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to 500 µg/mL inhibits cell death in chondrocytes induced by IL-1β. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-1β (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05). CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1β-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.
