Mechanism of anti-hyperplasia of mammary glands of Xihuang Pills blood-entering component based on UPLC-Q-TOF-MS and network pharmacology.
10.19540/j.cnki.cjcmm.20230719.202
- Author:
Qi-Rui FAN
1
;
Mei CHEN
1
;
Xiao-Yi DONG
1
;
Rui TAO
1
;
Jing-Rui WANG
1
;
Shun-Li XIA
1
;
Yong-Mei LAN
2
;
Tao HAN
3
Author Information
1. College of Pharmacy, Gansu University of Chinese Medicine Lanzhou 730000, China.
2. Northwest Minzu University Lanzhou 730030, China.
3. College of Pharmacy, Gansu University of Chinese Medicine Lanzhou 730000, China Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS;
Xihuang Pills;
human mammary epithelial cells MCF-10A;
hyperplasia of mammary glands;
molecular docking;
network pharmacology
- MeSH:
Humans;
Chromatography, High Pressure Liquid;
Molecular Docking Simulation;
Network Pharmacology;
Apoptosis;
Hyperplasia;
Drugs, Chinese Herbal/pharmacology*
- From:
China Journal of Chinese Materia Medica
2023;48(24):6749-6764
- CountryChina
- Language:Chinese
-
Abstract:
In this study, based on network pharmacology and molecular docking method, the mechanism of anti-hyperplasia of mammary glands of Xihuang Pills blood-entering components was explored, and the efficacy and key targets of Xihuang Pills blood-entering components were experimentally verified by MCF-10A proliferation model of human mammary epithelial cells. In order to clarify the material basis and mechanism of Xihuang Pills in realizing anti-hyperplasia of mammary glands, the blood-entering components of Xihuang Pills were qualitatively analyzed by UPLC-Q-TOF-MS, and 22 blood-entering components were identified. By taking the blood-entering components as the research object, the network pharmacology prediction and molecular docking verification were carried out, and finally, three key targets were screened out, namely JAK1, SRC, and CDK1. In vitro experiments show that Xihuang Pills can inhibit the proliferation of MCF-10A cells, promote the apoptosis of MCF-10A cells, and reduce the expression of JAK1, SRC, and CDK1 targets in cells. To sum up, Xihuang Pills can promote the apoptosis of mammary epithelial cells by regulating the expression of JAK1, SRC, and CDK1 and then play an anti-hyperplasia role, which provides an experimental basis for clarifying the material basis of Xihuang Pills for anti-hyperplasia effect.
