Functional characterization and enzymatic properties of flavonoid glycosyltransferase gene CtUGT49 in Carthamus tinctorius.
10.19540/j.cnki.cjcmm.20230809.101
- Author:
Xin-Bo CAI
1
;
Nan LIU
2
;
Jia LI
1
;
Rong LIU
1
;
Yun-Feng LUO
1
;
Yi-Feng ZHANG
3
;
Jia-Dian WANG
1
;
Xiao-Yi WU
1
;
Lu-Qi HUANG
3
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
2. School of Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
3. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Carthamus tinctorius;
enzymatic properties;
functional characterization;
glycosyltransferase
- MeSH:
Carthamus tinctorius/chemistry*;
Phylogeny;
Flavonoids/analysis*;
Glycosides/analysis*;
Glycosyltransferases/genetics*;
Anti-Inflammatory Agents;
Chalcones
- From:
China Journal of Chinese Materia Medica
2023;48(24):6624-6634
- CountryChina
- Language:Chinese
-
Abstract:
Carthami Flos, as a traditional blood-activating and stasis-resolving drug, possesses anti-tumor, anti-inflammatory, and immunomodulatory pharmacological activities. Flavonoid glycosides are the main bioactive components in Carthamus tinctorius. Glycosyltransferase deserves to be studied in depth as a downstream modification enzyme in the biosynthesis of active glycoside compounds. This study reported a flavonoid glycosyltransferase CtUGT49 from C. tinctorius based on the transcriptome data, followed by bioinformatic analysis and the investigation of enzymatic properties. The open reading frame(ORF) of the gene was 1 416 bp, encoding 471 amino acid residues with the molecular weight of about 52 kDa. Phylogenetic analysis showed that CtUGT49 belonged to the UGT73 family. According to in vitro enzymatic results, CtUGT49 could catalyze naringenin chalcone to the prunin and choerospondin, and catalyze phloretin to phlorizin and trilobatin, exhibiting good substrate versatility. After the recombinant protein CtUGT49 was obtained by hetero-logous expression and purification, the enzymatic properties of CtUGT49 catalyzing the formation of prunin from naringenin chalcone were investigated. The results showed that the optimal pH value for CtUGT49 catalysis was 7.0, the optimal temperature was 37 ℃, and the highest substrate conversion rate was achieved after 8 h of reaction. The results of enzymatic kinetic parameters showed that the K_m value was 209.90 μmol·L~(-1) and k_(cat) was 48.36 s~(-1) calculated with the method of Michaelis-Menten plot. The discovery of the novel glycosyltransferase CtUGT49 is important for enriching the library of glycosylation tool enzymes and provides a basis for analyzing the glycosylation process of flavonoid glycosides in C. tinctorius.
