Baimai Ointment relieves chronic pain induced by chronic compression of dorsal root ganglion in rats by regulating neuroactive ligand-receptor interaction and HIF-1 signaling pathway.
10.19540/j.cnki.cjcmm.20230721.401
- Author:
Fang-Ting ZHOU
1
;
Ying ZONG
2
;
Wu-Qiong HOU
2
;
Sen-Sen LI
2
;
Fei YANG
2
;
Li-Ting XU
1
;
Xia MAO
2
;
Yu-Dong LIU
2
;
Xiao-Hui SU
2
;
Hong-Ye WAN
3
;
Jing-Feng OUYANG
4
;
Qiu-Yan GUO
2
;
Wei-Jie LI
2
;
Zhen WANG
5
;
Chao WANG
2
;
Na LIN
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine Guangzhou 510405, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences Beijing 100700, China.
4. Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
5. Institute of Basic Theory of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Baimai Ointment;
lumbar disc herniation;
nerve ligands;
neuroinflammation;
relaxing sinew and activating collaterals
- MeSH:
Rats;
Mice;
Animals;
Chronic Pain/metabolism*;
Rats, Sprague-Dawley;
Ganglia, Spinal/metabolism*;
Ligands;
Signal Transduction;
Hyperalgesia/metabolism*;
Drugs, Chinese Herbal
- From:
China Journal of Chinese Materia Medica
2023;48(23):6457-6474
- CountryChina
- Language:Chinese
-
Abstract:
The Baimai Ointment with the effect of relaxing sinew and activating collaterals demonstrates a definite effect on Baimai disease with pain, spasm, stiffness and other symptoms, while the pharmacodynamic characteristics and mechanism of this agent remain unclear. In this study, a rat model of chronic compression of L4 dorsal root ganglion(CCD) was established by lumbar disc herniation, and the efficacy and mechanism of Baimai Ointment in the treatment of CCD were preliminarily explored by behavioral tests, side effect evaluation, network analysis, antagonist and molecular biology verification. The pharmacodynamic experiment indicated that Baimai Ointment significantly improved the pain thresholds(mechanical pain, thermal pain, and cold pain) and gait behavior of CCD model rats without causing tolerance or obvious toxic and side effects. Baimai Ointment inhibited the second-phase nociceptive response of mice in the formalin test, increased the hot plate threshold of normal mice, and down-regulated the expression of inflammatory cytokines in the spinal cord. Network analysis showed that Baimai Ointment had synergistic effect in the treatment of CCD and was related to descending inhibition/facilitation system and neuroinflammation. Furthermore, behavioral tests, Western blot, and immunofluorescence assay revealed that the pain-relieving effect of Baimai Ointment on CCD may be related to the regulation of the interaction between neuroactive ligand and receptors(neuroligands) such as CHRNA7, ADRA2A, and ADRB2, and the down-regulation of the expression of NOS2/pERK/PI3K, the core regulatory element of HIF-1 signaling pathway in spinal microglia. The findings preliminarily reveal the mechanism of relaxing sinew and activating collaterals of Baimai Ointment in the treatment of Baimai disease, providing a reference for the rational drug use and further research of this agent.
