Chemical constituents of Helleborus thibetanus.
10.19540/j.cnki.cjcmm.20230906.201
- Author:
Yu-Ze LI
1
;
Dong-Dong ZHANG
1
;
Wen-Li HUANG
1
;
Yi JIANG
1
;
Hua-Wei ZHANG
1
;
Chong DENG
1
;
Wei WANG
1
;
Jian-Li LIU
2
;
Xiao-Mei SONG
3
Author Information
1. School of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712046, China.
2. College of Life Science, Northwest University Xi'an 710069, China.
3. School of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712046, China Shaanxi Key Laboratory of Research and Application of "Taibai Qi Yao" Xianyang 712046, China.
- Publication Type:Journal Article
- Keywords:
Helleborus thibetanus;
chemical constituents;
isolation and identification;
new steroidal saponin
- MeSH:
Helleborus/chemistry*;
Molecular Structure;
Plant Roots/chemistry*;
Rhizome/chemistry*;
Magnetic Resonance Spectroscopy
- From:
China Journal of Chinese Materia Medica
2023;48(23):6408-6413
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Helleborus thibetanus were isolated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and semi-preparative RP-HPLC, and the structures of all compounds were identified by modern spectrographic technology(MS, NMR). The MTT method was used to measure the cytotoxicity of compounds 1-8. Twelve compounds were isolated from the roots and rhizomes of H. thibetanus and were identified as(25R)-22β,25-expoxy-26-[(O-β-D-glucopyranosyl)oxy]-1β,3β-dihydroxyfurosta-5-en(1), β-sitosterol myristate(2), β-sitosterol lactate(3), β-sitosterol 3-O-β-D-glucopyrannoside(4), 4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one(5), 1,3,5-trimethoxybenzene(6), 7,8-dimethylbenzo pteridine-2,4(1H,3H)-dione(7), 1H-indole-3-carboxylic acid(8), p-hydroxy cinnamic acid(9), lauric acid(10), n-butyl α-L-arabinofuranoside(11) and methyl-α-D-fructofuranoside(12), respectively. Among them, compound 1 is a new compound and named thibetanoside L; compounds 2, 5-8, 11 are first isolated from the family Ranunculaceae; compound 12 is isolated from the genus Helleborus for the first time. The results of MTT assay showed that the IC_(50) values of compounds 1-8 against HepG2 and HCT116 cells were greater than 100 μmol·L~(-1).
