Comparison on volatile components between Artemisiae Verlotori Folium and Artemisiae Argyi Folium based on GC-MS and chemometrics.
10.19540/j.cnki.cjcmm.20230901.101
- Author:
Jing DENG
1
;
Ting-Fen WU
1
;
Chu-Chu ZHONG
1
;
Zhi-Guo MA
2
;
Hui CAO
2
;
Ze-Bin LIN
3
;
Ying ZHANG
2
;
Meng-Hua WU
2
Author Information
1. Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Lingnan Resources Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Research Center for Traditional Chinese Medicine of Lingnan (Southern China), College of Pharmacy, Jinan University Guangzhou 510632, China.
2. Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Lingnan Resources Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Research Center for Traditional Chinese Medicine of Lingnan (Southern China), College of Pharmacy, Jinan University Guangzhou 510632, China Cao Hui's Studio for Inheritance of National Famous Traditional Chinese Medicine Experts Guangzhou 510632, China Cao Hui' Expert Workstation in Yunnan Province Kunming 655211, China.
3. Guangdong Geeching Biomedical Technology Co., Ltd.,/Healthcare Resource Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine Huizhou 516081, China.
- Publication Type:Journal Article
- Keywords:
Artemisiae Argyi Folium;
Artemisiae Verlotori Folium;
GC-MS;
hierarchical clustering analysis(HCA);
orthogonal partial least squares-discriminant analysis(OPLS-DA)
- MeSH:
Gas Chromatography-Mass Spectrometry;
Chemometrics;
Oils, Volatile;
Drugs, Chinese Herbal;
Plant Leaves;
Artemisia
- From:
China Journal of Chinese Materia Medica
2023;48(23):6334-6346
- CountryChina
- Language:Chinese
-
Abstract:
Artemisiae Argyi Folium is commonly used in clinical practice. Artemisiae Verlotori Folium, the dried leaves of Artemisia verlotorum, is often used as a folk substitute for Artemisiae Argyi Folium in Lingnan area. In this study, gas chromatography-triple quadrupole mass spectrometry(GC-MS) was used to detect the volatile oil components of 27 samples of Artemisiae Verlotori Folium and 13 samples of Artemisiae Argyi Folium, and the volatile components were compared between the two species. The internal standard method was combined with multi-reaction monitoring mode(MRM) to determine the content of six major volatile components. Hierarchical clustering analysis(HCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were carried out for the content data. The results showed that the Artemisiae Argyi Folium samples had higher content and more abundant volatile oils than the Artemisiae Verlotori Folium samples. Artemisiae Argyi Folium mainly had the components with lower boiling points, while Artemisiae Verlotori Folium mainly had the components with higher boiling points. Terpenoids were the main volatile components in Artemisiae Verlotori Folium(mainly sesquiterpenoids) and Artemisiae Argyi Folium(monoterpenoids). In addition, Artemisiae Argyi Folium had higher content of oxygen-containing derivatives than Artemisiae Verlotori Folium. Furthermore, the stoichiometric analysis showed that the two species could be distinguished by both HCA and OPLS-DA, indicating that the volatile components of the two were significantly different. This study can provide a scientific basis for the quality evaluation and data support for the local rational application of Artemisiae Verlotori Folium in Lingnan.
