Comprehensive identification of metabolites and metabolic characteristics of luteolin and kaempferol in Simiao Yong'an Decoction in rats by UHPLC-LTQ-Orbitrap MS/MS.
10.19540/j.cnki.cjcmm.20230621.201
- Author:
Man-Fang YANG
1
;
Hui LI
1
;
Xin-Rui XU
2
;
Qing-Yue ZHANG
2
;
Tian-Yu WANG
1
;
Lu FENG
2
;
Gui-Yang XIA
2
;
Zhan QIN
3
;
Bo NIE
1
Author Information
1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine Beijing 100700, China School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
2. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine Beijing 100700, China.
3. Guangdong Provincial Hospital of Chinese Medicine Zhuhai, Zhuhai 519015, China.
- Publication Type:Journal Article
- Keywords:
Simiao Yong′an Decoction;
kaempferol;
luteolin;
metabolism;
ultra-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry
- MeSH:
Rats;
Animals;
Tandem Mass Spectrometry/methods*;
Luteolin/analysis*;
Drugs, Chinese Herbal/chemistry*;
Kaempferols/analysis*;
Chromatography, High Pressure Liquid/methods*;
Molecular Docking Simulation
- From:
China Journal of Chinese Materia Medica
2023;48(22):6191-6199
- CountryChina
- Language:Chinese
-
Abstract:
Simiao Yong'an Decoction is a classic prescription for treating gangrene. Modern medical evidence has proven that Si-miao Yong'an Decoction has therapeutic effects on atherosclerosis(AS), vascular occlusion angeitides, and hypertension, while its pharmacodynamic mechanism remains unclear. The evidence of network pharmacology, molecular docking, literature review, and our previous study suggests that luteolin and kaempferol are two major flavonoids in Simiao Yong'an Decoction and can inhibit macrophage inflammation and exert anti-AS effects. However, due to lack of the metabolism studies in vivo, little is known about the metabolic characteristics of luteolin and kaempferol. This study employed ultra-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS/MS) and relevant software to identify the metabolites and metabolic pathways of luteolin and kaempferol in rat plasma, urine, and feces, after oral administration of luteolin and kaempferol, respectively. After the administration of luteolin, 10, 11, and 3 metabolites of luteolin were detected in the plasma, urine, and feces, respectively. After the administration of kaempferol, 9, 3, and 1 metabolites of kaempferol were detected in the plasma, urine, and feces, respectively. The metabolic pathways mainly involved methylation, glucuronidation, and sulfation. This study enriches the knowledge about the pharmacological mechanism of luteolin and kaempferol and supplies a reference for revealing the metabolic process of other flavonoids in Simiao Yong'an Decoction, which is of great significance for elucidating the pharmacological effects and effective substances of this decoction in vivo.
