Prediction of quality markers and medicinal value of sea buckthorn leaves based on network pharmacology, content determination, and activity evaluation.
10.19540/j.cnki.cjcmm.20230616.201
- Author:
Qian HE
1
;
Kai-Lin YANG
2
;
Xin-Yan WU
2
;
Bo ZHANG
3
;
Chun-Hong ZHANG
4
;
Chun-Nian HE
2
;
Pei-Gen XIAO
2
Author Information
1. Baotou Medical College Baotou 014060, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education Beijing 100193, China.
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education Beijing 100193, China.
3. Institute for Interdisciplinary Information Sciences, Tsinghua University Beijing 100193, China.
4. Baotou Medical College Baotou 014060, China.
- Publication Type:Journal Article
- Keywords:
fingerprinting;
network pharmacology;
quality markers;
sea buckthorn leaves
- MeSH:
Hippophae/chemistry*;
Network Pharmacology;
Molecular Docking Simulation;
Phosphatidylinositol 3-Kinases/metabolism*;
Flavonoids/analysis*;
Fruit/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(20):5487-5497
- CountryChina
- Language:Chinese
-
Abstract:
The leaves of sea buckthorn(Hippophae rhamnoides), considered as common food raw materials, have records of medicinal use and diverse pharmacological activities, showing a potential medicinal value. However, the active substances in the sea buckthorn leaves and their mechanisms of action remain unclear. In addition, due to the extensive source and large variety variations, the quality evaluation criteria of sea buckthorn leaves remain to be developed. To solve the problems, this study predicted the main active components, core targets, key pathways, and potential pharmacological effects of sea buckthorn leaves by network pharmacology and molecular docking. Furthermore, ultra-performance liquid chromatography with diode-array detection(UPLC-DAD) was employed to determine the content of active components and establish the chemical fingerprint, on the basis of which the quality markers of sea buckthorn leaves were predicted and then verified by the enzyme activity inhibition method. The results indicated that sea buckthorn leaves had potential therapeutic effects on a variety of digestive tract diseases, metabolic diseases, tumors, and autoimmune diseases, which were consistent with the ancient records and the results of modern pharmacological studies. The core targets of sea buckthorn leaves included PTPN11, AKT1, PIK3R1, ESR1, and SRC, which were mainly involved in the PI3K-AKT, MAPK, and HIF-1 signaling pathways. In conclusion, the active components of sea buckthorn leaves are associated with the rich flavonoids and tannins, among which quercitrin, narcissoside, and ellagic acid can be used as the quality markers of sea buckthorn leaves. The findings provide a reference for the quality control and further development and utilization of sea buckthorn leaves as medicinal materials.
