Linderae Radix water extract treats diarrhea-predominant irritable bowel syndrome in rats: a serum metabolomics study.
10.19540/j.cnki.cjcmm.20230614.701
- Author:
Tao LIU
1
;
Meng-Ling WU
2
;
Guo-Yan DENG
2
;
Yang HE
2
;
Yi-Ran HE
2
;
Gui-Ming DENG
2
;
Lin-Qi OUYANG
3
Author Information
1. the First Hospital of Hunan University of Chinese Medicine Changsha 410007, China Changsha Hospital, Xiangya School of Medicine, Central South University Changsha 410005, China.
2. the First Hospital of Hunan University of Chinese Medicine Changsha 410007, China.
3. the First Hospital of Hunan University of Chinese Medicine Changsha 410007, China School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
Linderae Radix water extract;
diarrhea-predominant irritable bowel syndrome;
metabonomics;
potential biomarkers;
riboflavin metabolism;
tryptophan metabolism
- MeSH:
Rats;
Male;
Animals;
Irritable Bowel Syndrome/metabolism*;
Water;
Chromatography, Liquid;
Tryptophan;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry;
Diarrhea/drug therapy*;
Biomarkers;
Riboflavin
- From:
China Journal of Chinese Materia Medica
2023;48(19):5356-5364
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the mechanism of Linderae Radix water extract(LRWE) in the prevention and treatment of diarrhea-predominant irritable bowel syndrome(IBS-D) based on serum metabolomics. Eighteen 2-week-old male SD rats were randomized into control, IBS-D model, and LRWE groups. The rats in other groups except the control group received gavage of senna concentrate combined with restraint stress for the modeling of IBS-D. The rats in the LRWE group were administrated with LRWE(5.4 g·kg~(-1)) by gavage, and those in the control and IBS-D model groups with an equal volume of distilled water for a total of 14 days. The visceral sensitivity was evaluated by the abdominal withdrawal reflex(AWR) score, and the degree of diarrhea was assessed by the fecal water content(FWC). The morphological changes of the colon and the morphology and number of goblet cells were observed by hematoxylin-eosin(HE) and periodic acid-schiff(PAS) staining, respectively. Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was used for the screening of the potential biomarkers in the rat serum and their related metabolic pathways. The results showed that LRWE reduced the AWR score, decreased FWC, and alleviated visceral sensitivity and diarrhea symptoms in IBS-D rats. HE and PAS staining showed that LRWE mitigated low-grade intestinal inflammation and increased the number of mature secretory goblet cells in the colonic epithelium of IBS-D rats. A total of 25 potential biomarkers of LRWE in treating IBS-D were screened out in this study, which were mainly involved in riboflavin, tryptophan, glycine, serine and threonine metabolism, glyoxylate and dicarboxylate metabolism, and cysteine and methionine metabolism. The regulatory effects were the most significant on the riboflavin and tryptophan metabolism pathways. LRWE may alleviate the visceral hypersensitivity by promoting energy metabolism and amino acid metabolism, enhancing intestinal barrier function, and improving intestinal immune function in IBS-D rats.
