Comparison on anti-inflammatory activity of Gynostemma pentaphyllum processed with different methods.
10.19540/j.cnki.cjcmm.20230711.303
- Author:
Shu-Yang XU
1
;
Zi-Qing YANG
1
;
Fei TENG
2
;
Xun-Jiang WANG
3
;
Qin HUANG
4
;
De-Zhen JIN
4
;
Min LI
2
;
Shou-Jin LIU
5
;
Zheng-Tao WANG
3
;
Li-Li DING
3
;
Jing-Jing ZHU
2
Author Information
1. Anhui University of Chinese Medicine Hefei 230012, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Shanghai Key Laboratory of Compound Medicine, MOE Key Laboratory of Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Shanghai Key Laboratory of Compound Medicine, MOE Key Laboratory of Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
4. Pingli Tea and Gynostemma pentaphyllum Development Center Ankang 725500, China.
5. Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
acute lung injury;
anti-inflammatory activity;
gypenosides;
processing method;
rare saponins;
sugar chain length
- MeSH:
Male;
Mice;
Animals;
Tumor Necrosis Factor-alpha/metabolism*;
Gynostemma;
Mice, Inbred C57BL;
Lung;
Anti-Inflammatory Agents/metabolism*;
Interleukin-6/metabolism*;
Interleukin-1beta/metabolism*;
Lipopolysaccharides/pharmacology*
- From:
China Journal of Chinese Materia Medica
2023;48(19):5235-5243
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the effects of Gynostemma pentaphyllum dried with two different methods(air drying and heating) on inflammation in acute lung injury(ALI) mice in vivo and in vitro. Lipopolysaccharide(LPS) was sprayed into the airway of wild type C57BL/6J male mice to establish the model, and the drug was injected into the tail vein 24 h after modeling. Lung function, lung tissue wet/dry weight(W/D) ratio, the total protein concentration, interleukin 6(IL-6), IL-1β, and tumor necrosis factor-α(TNF-α) in the bronchoalveolar lavage fluid(BALF), and pathological changes of the lung tissue were used to evaluate the effects of different gypenosides on ALI mice. The results showed that total gypenosides(YGGPs) and the gypenosides substituted with one or two glycosyl(GPs_(1-2)) in the air-dried sample improved the lung function, significantly lowered the levels of IL-1β and TNF-α in BALF, and alleviated the lung inflammation of ALI mice. Moreover, GPs_(1-2) had a more significant effect on inhibiting NO release in RAW264.7 cells. This study showed that different drying methods affected the anti-inflammatory activity of G. pentaphyllum, and the rare saponins in the air-dried sample without heating had better anti-inflammatory activity.
