Morin induces autophagy and apoptosis in hepatocellular carcinoma cells through Akt/mTOR/STAT3 pathway.
10.19540/j.cnki.cjcmm.20230413.704
- Author:
Xin-Yue ZHAO
1
;
Ying-Ying TIAN
1
;
Chuang LIU
1
;
Yi-Lin LI
1
;
Ying-Nan LYU
1
;
Shang-Yue YU
1
;
Shi-Qiu TIAN
1
;
Hai-Luan PEI
1
;
Ze-Ping ZUO
2
;
Zhi-Bin WANG
3
Author Information
1. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China.
2. Beijing Tongrentang Technologies Co.,Ltd.,Pharmaceutical Factory Beijing 100079,China.
3. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China Beijing Tongrentang Technologies Co.,Ltd.,Pharmaceutical Factory Beijing 100079,China.
- Publication Type:Journal Article
- Keywords:
Akt/mTOR/STAT3 pathway;
apoptosis;
autophagy;
hepatocellular carcinoma;
morin;
proliferation
- MeSH:
Humans;
Proto-Oncogene Proteins c-akt/metabolism*;
Carcinoma, Hepatocellular/pathology*;
Liver Neoplasms/pathology*;
TOR Serine-Threonine Kinases/metabolism*;
Apoptosis;
Autophagy;
Cell Proliferation;
Cell Line, Tumor;
STAT3 Transcription Factor/metabolism*
- From:
China Journal of Chinese Materia Medica
2023;48(16):4475-4482
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effect and mechanism of morin in inducing autophagy and apoptosis in hepatocellular carcinoma cells through the protein kinase B(Akt)/mammalian target of rapamycin(mTOR)/signal transducer and activator of transcription protein 3(STAT3) pathway. Human hepatocellular carcinoma SK-HEP-1 cells were stimulated with different concentrations of morin(0, 50, 100, 125, 200, and 250 μmol·L~(-1)). The effect of morin on the viability of SK-HEP-1 cells was detected by Cell Counting Kit-8(CCK-8). The effect of morin on the proliferation and apoptosis of SK-HEP-1 cells was investigated using colony formation assay, flow cytometry, and BeyoClick~(TM) EdU-488 with different concentrations of morin(0, 125, and 250 μmol·L~(-1)). The changes in the autophagy level of cells treated with morin were examined by transmission electron microscopy and autophagy inhibitors. The impact of morin on the expression levels of proteins related to the Akt/mTOR/STAT3 pathway was verified by Western blot. Compared with the control group, the morin groups showed decreased viability of SK-HEP-1 cells in a time-and concentration-dependent manner, increased number of apoptotic cells, up-regulated expression level of apoptosis marker PARP, up-regulated phosphorylation level of apoptosis-regulating protein H2AX, decreased number of positive cells and the colony formation rate, an upward trend of expression levels of autophagy-related proteins LC3-Ⅱ, Atg5, and Atg7, and decreased phosphorylation levels of Akt, mTOR, and STAT3. These results suggest that morin can promote apoptosis, inhibit proliferation, and induce autophagy in hepatocellular carcinoma cells, and its mechanism of action may be related to the Akt/mTOR/STAT3 pathway.
