Therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma in treatment of gastric ulcer based on network pharmacology and animal experiment.
10.19540/j.cnki.cjcmm.20230320.401
- Author:
Jing-Xian FANG
1
;
Lian ZHANG
1
;
Jing LI
1
;
Han-Rui ZHANG
1
;
Dan LIU
1
;
Jing NIE
2
;
Xiao-Chuan YE
1
Author Information
1. Hubei Key Laboratory of Resource Science and Chemistry in Chinese Medicine,Pharmacy Faculty,Hubei University of Chinese Medicine Wuhan 430065,China.
2. Hubei Center for ADR Monitoring Wuhan 430071,China.
- Publication Type:Journal Article
- Keywords:
Bletillae Rhizoma;
PI3K/AKT;
gastric ulcer;
network pharmacology;
non-polysaccharide fraction
- MeSH:
Animals;
Mice;
Stomach Ulcer/drug therapy*;
Proto-Oncogene Proteins c-akt;
Animal Experimentation;
Network Pharmacology;
Phosphatidylinositol 3-Kinases;
Tandem Mass Spectrometry;
Vascular Endothelial Growth Factor A;
Tumor Necrosis Factor-alpha;
Molecular Docking Simulation;
Drugs, Chinese Herbal/pharmacology*
- From:
China Journal of Chinese Materia Medica
2023;48(16):4446-4458
- CountryChina
- Language:Chinese
-
Abstract:
The present study aimed to explore the therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma in the treatment of gastric ulcer by network pharmacology and animal experiments. UPLC-Q-TOF-MS/MS was employed to chara-cterize the chemical components of non-polysaccharide fraction of Bletillae Rhizoma, and the common targets of Bletillae Rhizoma and gastric ulcer were screened out by network pharmacology. The "drug-component-target-disease" network was constructed. Protein-protein interaction(PPI) network was established by STRING. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed based on Matescape database to predict the therapeutic effect and mechanism of Bletillae Rhizoma. Finally, the gastric ulcer model was induced in mice by alcohol to verify the therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma on gastric ulcer. Forty-seven chemical components were identified from non-polysaccharide fraction of Bletillae Rhizoma, among which gymnoside Ⅰ, gymnoside Ⅱ, militarine, bletilloside A, and shancigusin I might be the main active components of non-polysaccharide fraction of Bletillae Rhizoma against gastric ulcer. PPI network analysis revealed core targets such as albumin(ALB), serine/threonine kinase 1(AKT1), tumor necrosis factor(TNF), and epidermal growth factor receptor(EGFR). The KEGG enrichment analysis showed that non-polysaccharide fraction of Bletillae Rhizoma mainly exerted the therapeutic effect by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT) signaling pathway, mitogen-activated protein kinase(MAPK) signaling pathway, and Ras signaling pathway. The results of animal experiments showed that non-polysaccharide fraction of Bletillae Rhizoma could significantly improve alcohol-induced ulceration in mice to increase ulcer inhibition rate, decrease the levels of TNF-α, interleukin(IL)-1β, IL-6, vasoactive intestinal peptide(VIP), and thromboxane B2(TXB2), elevated the le-vels of IL-10, prostaglandin E2(PGE2), epidermal growth factor(EGF), and vascular endothelial growth factor(VEGF), down-re-gulate the protein levels of PI3K and AKT, and up-regulate the protein levels of p-PI3K and p-AKT. This study indicates that Bletillae Rhizoma may play a role in the treatment of gastric ulcer through multiple components, targets, and pathways and verifies partial prediction results of network pharmacology. The findings of this study provide a scientific and experimental basis for clinical application.
