Structural characterization of PCP-Ⅰ from Poria as vaccine adjuvant and its hydrolytic oligosaccharide.
10.19540/j.cnki.cjcmm.20230124.201
- Author:
Jia-Nan GU
1
;
Gui-Xin LIU
2
;
Shuai LI
2
;
Hao MA
2
;
Jun-Jie SHAN
1
Author Information
1. Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine Shanghai 201203,China Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences Beijing 100850,China.
2. Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences Beijing 100850,China.
- Publication Type:Journal Article
- Keywords:
Poria;
chemical structure;
oligosaccharide;
polysaccharide;
vaccine adjuvant
- MeSH:
Adjuvants, Vaccine;
Poria;
Hydrolysis;
Spectroscopy, Fourier Transform Infrared;
Galactose;
Influenza A Virus, H1N1 Subtype;
Polysaccharides/chemistry*;
Oligosaccharides;
Carbon
- From:
China Journal of Chinese Materia Medica
2023;48(16):4429-4437
- CountryChina
- Language:Chinese
-
Abstract:
Poria is an important medical herb in clinic. The authors isolated a polysaccharide(PCP-Ⅰ) from Poria in previous studies, which is composed of galactose, mannose, fucose and glucose. PCP-Ⅰ exhibited significant adjuvant effects on H1N1 influenza vaccine, hepatitis B surface antigen and anthrax protective antigen, and its adjuvant activity was stronger than aluminium adjuvant. However, little is known about the chemical structure of PCP-Ⅰ at present. In this study, weak acid hydrolysis was used to obtain the backbone oligosaccharide of PCP-Ⅰ. Then periodate oxidation, Smith degradation, methylation analysis, Fourier transform infrared spectroscopy(FT-IR), nuclear magnetic resonance(NMR) and gas chromatography-mass spectrometry(GC-MS) were performed to investigate the chemical structural features of PCP-Ⅰ and its hydrolytic oligosaccharide(PCP-Ⅰ-hy-1). These results suggested that the backbone of PCP-Ⅰ was composed of galactose with α anomeric carbon and β anomeric carbon. The linking residues of galactan are(1→),(l→6) and(1→2,6).
