Prediction and analysis of Q-markers of Elephantopus scaber based on its UPLC fingerprint, content determination of components, and in vitro a nti-tumor activity.
10.19540/j.cnki.cjcmm.20230526.201
- Author:
Can-Chao JIA
1
;
Ling-Jie LI
2
;
Zhi-Hao ZENG
1
;
Rui-Yin TANG
1
;
De-Zheng JIA
1
;
Min-Juan YANG
1
;
Jin-Yan QIU
1
;
Dong-Mei LI
1
;
Can-Hui XIE
1
;
Guang-Ying WU
1
;
Yang-Xue LI
3
;
Jie-Yi JIANG
3
;
Hong HUANG
4
;
Guan-Lin XIAO
3
;
Da-Ke CAI
5
;
Xiao-Li BI
3
Author Information
1. the Fifth Clinical Medicine School,Guangzhou University of Chinese Medicine Guangzhou 510405,China.
2. the Fifth Clinical Medicine School,Guangzhou University of Chinese Medicine Guangzhou 510405,China Department of Pharmacy,Guangdong Provincial People's Hospital Guangzhou 510010,China.
3. Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology Guangzhou 510095,China Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine Guangzhou 510095,China.
4. Guangzhou Baiyun Institutes for Food and Drug Control Guangzhou 510405,China.
5. Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology Guangzhou 510095,China Department of Pharmacy,Guangdong Provincial People's Hospital Guangzhou 510010,China.
- Publication Type:Journal Article
- Keywords:
Elephantopus scaber;
content determination;
fingerprint;
inhibitory activity;
non-small cell lung cancer
- MeSH:
Humans;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Asteraceae/chemistry*;
Lung Neoplasms/drug therapy*
- From:
China Journal of Chinese Materia Medica
2023;48(16):4421-4428
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to provide scientific evidence for predicting quality markers(Q-markers) of Elephantopus scaber by establishing UPLC fingerprint of E. scaber from different geographical origins and determining the content of 13 major components, as well as conducting in vitro anti-cancer activity investigation of the main components. The chromatographic column used was Waters CORTECS UPLC C_(18)(2.1 mm×150 mm, 1.6 μm), and the mobile phase consisted of acetonitrile and 0.1% formic acid solution(gradient elution). The column temperature was set at 30 ℃, and the flow rate was 0.2 mL·min~(-1). The injection volume was 1 μL, and the detection wavelength was 240 nm. The UPLC fingerprint of E. scaber was fitted using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition) to determine common peaks, evaluate similarity, identify and determine the content of major components. The CCK-8 assay was used to explore the inhibitory effect of the main components on the proliferation of lung cancer cells. The results showed that in the established UPLC fingerprint of E. scaber, 35 common peaks were identified. Thirteen major components, including neochlorogenic acid(peak 1), chlorogenic acid(peak 2), cryptochlorogenic acid(peak 3), caffeic acid(peak 4), schaftoside(peak 6), galuteolin(peak 9), isochlorogenic acid B(peak 10), isochlorogenic acid A(peak 12), isochlorogenic acid C(peak 18), deoxyelephantopin(peak 28), isodeoxyelephantopin(peak 29), isoscabertopin(peak 31), and scabertopin(peak 32) were identified and quantified, and a quantitative analysis method was established. The results of the in vitro anti-cancer activity study showed that deoxyelephantopin, isodeoxyelephantopin, isoscabertopin, and scabertopin in E. scaber exhibited inhibition rates of lung cancer cell proliferation exceeding 80% at a concentration of 10 μmol·L~(-1), higher than the positive drug paclitaxel. These results indicate that the fingerprint of E. scaber is highly characteristic, and the quantitative analysis method is accurate and stable, providing references for the research on quality standards of E. scaber. Four sesquiterpene lactones in E. scaber show significant anti-cancer activity and can serve as Q-markers for E. scaber.
