Effect of acetylalkannin from Arnebia euchroma on proliferation, migration, and invasion of human melanoma A375 cells.
10.19540/j.cnki.cjcmm.20230509.401
- Author:
Ying-Ying KANG
1
;
Qian QIAN
1
;
Ya YANG
1
;
Ying YANG
1
;
Fang XU
1
;
Min LI
1
;
Jian-Guang LI
2
Author Information
1. College of Pharmacy of Xinjiang Medical University Urumqi 830011, China.
2. Xinjiang University of Science and Technology Korla 841000, China.
- Publication Type:Journal Article
- Keywords:
Arnebia euchroma;
acetylalkannin;
invasion;
melanoma A375 cells;
migration
- MeSH:
Humans;
Matrix Metalloproteinase 2/metabolism*;
Glycogen Synthase Kinase 3 beta/metabolism*;
beta Catenin/metabolism*;
Vimentin/metabolism*;
Matrix Metalloproteinase 9/metabolism*;
Cell Line, Tumor;
Wnt Signaling Pathway;
Cadherins/genetics*;
Melanoma/genetics*;
Cyclin D/metabolism*;
Cell Proliferation;
Boraginaceae/genetics*;
RNA, Messenger;
Cell Movement
- From:
China Journal of Chinese Materia Medica
2023;48(18):5049-5055
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to explore the effect and mechanism of acetylalkannin from Arnebia euchroma on the proliferation, migration, and invasion of human melanoma A375 cells. A375 cells were divided into a blank group, and low-, medium-, and high-dose acetylalkannin groups(0.5, 1.0, and 2.0 μmol·L~(-1)). The MTT assay was used to detect cell proliferation. Cell scratch and transwell migration assays were used to detect cell migration ability, and the transwell invasion assay was used to detect cell invasion ability. Western blot was used to detect the protein expression of migration and invasion-related N-cadherin, vimentin, matrix metalloproteina-se-9(MMP-9), and Wnt/β-catenin pathway-related Wnt1, Axin2, glycogen synthase kinase-3β(GSK-3β), phosphorylated GSK-3β(p-GSK-3β), β-catenin, cell cycle protein D_1(cyclin D_1), and p21. Real-time fluorescence-based quantitative polymerase chain reaction(real-time PCR) was used to detect the mRNA expression of E-cadherin, matrix metalloproteinase-2(MMP-2), N-cadherin, vimentin, β-catenin, snail-1, and CD44. MTT results showed that the cell inhibition rates in the acetylalkannin groups significantly increased as compared with that in the blank group(P<0.01). The results of cell scratch and transwell assays showed that compared with the blank group, the acetylalkannin groups showed reduced cell migration and invasion, and migration and invasion rates(P<0.05, P<0.01) and weakened horizontal and vertical migration and invasion abilities. Western blot results showed that compared with the blank group, the high-dose acetylalkannin group showed increased expression of Axin2 protein(P<0.05), and decreased expression of N-cadherin, vimentin, MMP-9, Wnt1, p-GSK-3β, β-catenin, cyclin D_1, and p21 proteins(P<0.05, P<0.01). The expression of GSK-3β protein did not change significantly. PCR results showed that the overall trend of MMP-2, N-cadherin, vimentin, β-catenin, snail-1, and CD44 mRNA expression was down-regulated(P<0.01), and the expression of E-cadherin mRNA increased(P<0.01). Acetylalkannin can inhibit the proliferation, migration, and invasion of human melanoma A375 cells, and its mechanism of action may be related to the regulation of Wnt/β-catenin signaling pathway.
