Screening and molecular identification of endophytic fungi promoting accumulation of flavonoids in callus of Scutellaria baicalensis.
10.19540/j.cnki.cjcmm.20230606.101
- Author:
Xin ZHANG
1
;
Xiao-Xuan CUI
1
;
Yu-Guang ZHENG
2
;
Chun-Yan SU
1
Author Information
1. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China.
2. Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China Hebei Chemical and Pharmaceutical College Shijiazhuang 050026, China.
- Publication Type:Journal Article
- Keywords:
Scutellaria baicalensis;
elicitors;
endophytic fungi;
flavonoids
- MeSH:
Scutellaria baicalensis;
Plant Roots;
Flavanones;
Flavonoids
- From:
China Journal of Chinese Materia Medica
2023;48(18):4974-4980
- CountryChina
- Language:Chinese
-
Abstract:
To screen and identify the endophytic fungal strains that could promote the accumulation of flavonoids in the callus of Scutellaria baicalensis. Seventeen endophytic fungal strains from S. baicalensis were used to prepare mycelium elicitors and fermentation broth elicitors. Their effects on flavonoid accumulation in S. baicalensis callus were then determined. The results showed that the fermentation broth elicitors of two strains(CL79, CL105) promoted the accumulation of flavonoids. The fermentation broth elicitor of CL79 significantly promoted accumulation of baicalin, wogonoside, baicalein, and wogonin, with the maximum levels increased by 37.8%, 40.4%, 44.7%, and 42.2%(vs. blank), respectively. Similarly, the fermentation broth elicitor of CL105 significantly promoted the accumulation of baicalin, wogonoside, baicalein, and wogonin, with the maximum levels increased by 78.1%, 140.9%, 275.6%, and 208.5%(vs. blank), respectively. CL79 was identified as Alternaria alternata, and CL105 as Fusarium solani. The fermentation broth elicitors of A. alternata CL79 and F. solani CL105 were able to promote the flavonoid accumulation in the callus of S. baicalensis, which enriched the resources of endophytic fungi and provided candidate strains for the development of microbial fertili-zers for improving the quality of S. baicalensis.
