Dead heart of pith-decayed Scutellariae Radix: a study based on multi-omics.
10.19540/j.cnki.cjcmm.20230414.101
- Author:
Jin-Xiu QIAN
1
;
Ya-Peng WANG
1
;
Huai-Zhu LI
2
;
Yan-Meng LIU
1
;
Yi-Han WANG
1
;
Li-Ping KANG
1
;
Tie-Gui NAN
1
;
Jin-Fu TANG
1
;
Zhi-Lai ZHAN
1
Author Information
1. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. College of Chemistry and Chemical Engineering, Xianyang Normal University Xianyang 712000, China.
- Publication Type:Journal Article
- Keywords:
Scutellariae Radix;
dead heart;
metabolome;
programmed apoptosis;
transcriptome
- MeSH:
Drugs, Chinese Herbal/chemistry*;
Scutellaria baicalensis/chemistry*;
Glucuronides;
Multiomics;
Flavonoids/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(17):4634-4646
- CountryChina
- Language:Chinese
-
Abstract:
Dead heart is an important trait of pith-decayed Scutellariae Radix. The purpose of this study was to clarify the scientific connotation of the dead heart using multi-omics. Metabolomics and transcriptomics combined with multivariate statistical analysis such as principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) were used to systematically compare the differences in chemical composition and gene expression among phloem, outer xylem and near-dead xylem of pith-decayed Scutella-riae Radix. The results revealed significant differences in the contents of flavonoid glycosides and aglycones among the three parts. Compared with phloem and outer xylem, near-dead xylem had markedly lowered content of flavonoid glycosides(including baicalin, norwogonin-7-O-β-D-glucuronide, oroxylin A-7-O-β-D-glucuronide, and wogonoside) while markedly increased content of aglycones(including 3,5,7,2',6'-pentahydroxy dihydroflavone, baicalin, wogonin, and oroxylin A). The differentially expressed genes were mainly concentrated in KEGG pathways such as phenylpropanoid metabolism, flavonoid biosynthesis, ABC transporter, and plant MAPK signal transduction pathway. This study systematically elucidated the material basis of the dead heart of pith-decayed Scutellariae Radix with multiple growing years. Specifically, the content of flavonoid aglycones was significantly increased in the near-dead xylem, and the gene expression of metabolic pathways such as flavonoid glycoside hydrolysis, interxylary cork development and programmed apoptosis was significantly up-regulated. This study provided a theoretical basis for guiding the high-quality production of pith-decayed Scutellariae Radix.
