Serum metabolomics study of Psoraleae Fructus in improving learning and memory ability of APP/PS1 mice.
10.19540/j.cnki.cjcmm.20230303.401
- Author:
Jia-Ming GU
1
;
Hui XUE
2
;
Ao XUE
1
;
Jing JIANG
1
;
Fang GENG
3
;
Ji-Hui ZHAO
4
;
Bo YANG
1
;
Ning ZHANG
2
Author Information
1. School of Pharmacy, Heilongjiang University of Chinese Medicine Harbin 150040, China.
2. School of Pharmacy, Heilongjiang University of Chinese Medicine Harbin 150040, China Jiamusi College, Heilongjiang University of Chinese Medicine Jiamusi 154007, China.
3. College of Chemistry and Chemical Engineering, Harbin Normal University Harbin 150025, China.
4. School of Pharmaceutical Sciences, Hunan University of Medicine Huaihua 418000, China.
- Publication Type:Journal Article
- Keywords:
APP/PS1;
Alzheimer′s disease;
Psoraleae Fructus;
energy metabolism;
metabolomics
- MeSH:
Mice;
Animals;
Amyloid beta-Protein Precursor/genetics*;
Mice, Transgenic;
Arachidonic Acid;
Tryptophan;
Mice, Inbred C57BL;
Alzheimer Disease/genetics*;
Maze Learning;
Glycerophospholipids;
Disease Models, Animal;
Amyloid beta-Peptides/metabolism*
- From:
China Journal of Chinese Materia Medica
2023;48(15):4039-4045
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the mechanism of Psoraleae Fructus in improving the learning and memory ability of APP/PS1 mice by serum metabolomics, screen the differential metabolites of Psoraleae Fructus on APP/PS1 mice, and reveal its influence on the metabolic pathway of APP/PS1 mice. Thirty 3-month-old APP/PS1 mice were randomly divided into a model group and a Psoraleae Fructus extract group, and another 15 C57BL/6 mice of the same age were assigned to the blank group. The learning and memory ability of mice was evaluated by the Morris water maze and novel object recognition tests, and metabolomics was used to analyze the metabolites in mouse serum. The results of the Morris water maze test showed that Psoraleae Fructus shortened the escape latency of APP/PS1 mice(P<0.01), and increased the number of platform crossing and residence time in the target quadrant(P<0.01). The results of the novel object recognition test showed that Psoraleae Fructus could improve the novel object recognition index of APP/PS1 mice(P<0.01). Eighteen differential metabolites in serum were screened out by metabolomics, among which the levels of arachidonic acid, tryptophan, and glycerophospholipid decreased after drug administration, while the levels of glutamyltyrosine increased after drug administration. The metabolic pathways involved included arachidonic acid metabolism, glycerophospholipid metabolism, tryptophan metabolism, linoleic acid metabolism, α-linolenic acid metabolism, and glycerolipid metabolism. Therefore, Psoraleae Fructus can improve the learning and memory ability of APP/PS1 mice, and its mechanism may be related to the effects in promoting energy metabolism, reducing oxidative damage, protecting central nervous system, reducing neuroinflammation, and reducing Aβ deposition. This study is expected to provide references for Psoraleae Fructus in the treatment of Alzheimer's disease(AD) and further explain the mechanism of Psoraleae Fructus in the treatment of AD.
