Comparative study on serum transitional components of Huatan Jiangqi Capsules in rats under pathological and physiological status.
10.19540/j.cnki.cjcmm.20191204.202
- Author:
Chen-Hui LI
1
;
Wei FANG
1
;
Min ZHANG
1
;
Yuan-Yuan ZHOU
1
;
Ling-Ling XU
1
;
He-Ping HUANG
1
;
Dian-Lei WANG
2
;
Ze-Geng LI
3
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
2. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China Anhui Provincial Key Laboratory of Chinese Medicinal Formula Hefei 230012, China.
3. First Affiliated Hospital of Anhui University of Chinese Medicine Hefei 230031, China.
- Publication Type:Journal Article
- Keywords:
Huatan Jiangqi Capsules;
UPLC-Q-TOF-MS;
chronic obstructive pulmonary disease;
metabolic reaction;
transitional components
- MeSH:
Animals;
Capsules;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/pharmacokinetics*;
Mass Spectrometry;
Pulmonary Disease, Chronic Obstructive/drug therapy*;
Rats;
Serum/chemistry*
- From:
China Journal of Chinese Materia Medica
2020;45(6):1440-1451
- CountryChina
- Language:Chinese
-
Abstract:
The differences of transitional components and metabolic processes of Huatan Jiangqi Capsules(HTJQ) in rats under normal physiological and pathological conditions of COPD were analyzed by UPLC-Q-TOF-MS. The rat COPD model was established by passive smoking and intratracheal instillation of lipopolysaccharide. After the normal and COPD model rats were douched with HTJQ, the blood was collected from hepatic portal vein and the drug-containing serum samples were prepared by methanol precipitation of protein. Then, 10 batches of drug-containing serum samples of HTJQ were prepared and analyzed by UPLC serum fingerprint to evaluate the quality and stability of drug-containing serum samples. UPLC-Q-TOF-MS was used to collect the mass spectrometric information of the transitional components. Twenty-eight transitional components of HTJQ in normal rats and 25 transitional components of HTJQ in COPD model rats were identified by UPLC-Q-TOF-MS. Under pathological and physiological conditions, there were not only the same transitional components in rat serum, but also corresponding differences. Further studies showed that there were also differences in the metabolic process of transitional components between the two conditions. In normal rats, most of the metabolic types of transitional components were phase I reactions. In COPD model rats, phase Ⅰ reactions decreased and phase Ⅱ reactions increased correspondingly. With UPLC-Q-TOF-MS technology, the differences of transitional components and the metabolism process of HTJQ in rats under normal physiological and pathological conditions were analyzed. The results showed that types of transitional components and the activity of some metabolic enzymes would be changed in COPD pathological state, which would affect the metabolic process of bioactive components in vivo. It laid a foundation for further elucidating the metabolic process and pharmacodynamic substance basis of HTJQ.
