Study on protective effect of water extract from Sabia parviflora on liver injury in mice induced by acetaminophen.
10.19540/j.cnki.cjcmm.20191112.407
- Author:
Jian-Qiao LI
1
;
Wen-Feng HUANG
1
;
Hai-Bo HE
2
;
Dong-Xiao ZHAO
3
;
Jian-Zhong HU
3
;
Shun-Guang LU
3
;
Jia-Xin YE
1
;
Kun ZOU
2
Author Information
1. Hubei Key Laboratory of Natural Products Research and Development, Medical College of China Three Gorges University Yichang 443002, China.
2. Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences of China Three Gorges University Yichang 443002, China.
3. Plant Development and Management Center of Soil and Water Conservation, Ministry of Water Resources Beijing 100383, China.
- Publication Type:Journal Article
- Keywords:
Sabia parviflora;
acetaminophen;
cell apoptosis;
drug-induced liver injury;
oxidative
- MeSH:
Acetaminophen/toxicity*;
Alanine Transaminase/metabolism*;
Animals;
Aspartate Aminotransferases/metabolism*;
Chemical and Drug Induced Liver Injury/drug therapy*;
Liver/enzymology*;
Malondialdehyde/analysis*;
Mice;
Oxidative Stress;
Plant Extracts/pharmacology*;
Superoxide Dismutase/metabolism*
- From:
China Journal of Chinese Materia Medica
2020;45(6):1433-1439
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to observe the protective effect of water extract from Sabia parviflora on mice with acute liver injury induced by acetaminophen, and investigate its possible mechanism. Fifty-eight Kunming mice were divided into 6 groups, 8 in the normal group, 10 in the model group, 10 in the biphenyl diester group, and 10 each in the low, medium and high dose groups. After adaptive feeding for one week, the mice in normal group were intragastrically administered with an equal volume of 0.5% sodium carboxymethylcellulose sodium(CMC-Na), and the mice in other groups were intragastrically administered with corresponding drugs at 20 mL·kg~(-1) once a day. Then acetaminophen(200 mg·kg~(-1)) was administered after the above drug administration except the normal group. The behavior and signs of the experimental animals were observed every day and the samples were taken for experiments on the next day of the final administration. The liver mass and mass index were calculated. The blood was collected from the abdominal aorta and centrifuged to obtain the serum for detecting aspartate aminotransferase(AST) activity and alanine aminotransferase(ALT) activity. The liver tissue homogenate was used to detect superoxide dismutase(SOD) activity, glutathione(glutathione, r-glutamyl cysteingl+glycine, GSH) activity and malondialdehyde(MDA) content. Liver tissue was analyzed for histological analysis. The results showed that S. parviflora could alleviate the lipid peroxidation damage in the liver caused by acetaminophen, reduce the ALT and AST activities in serum, increase the levels of SOD and GSH in liver tissue, decrease the content of MDA in liver tissue, and inhibit the apoptosis. S. parviflora could also improve the live histopathological profile, protect liver cells and restore liver function. Among them, the high dose had the most significant effect and showed dose-effect relationship. This study indicated that S. parviflora had a significant protective effect on acetaminophen-induced liver injury in mice, and its mechanism may be related to its anti-oxidation effect and inhi-bitory effect on apoptosis.
