Simultaneous determination of six major isosteroidal alkaloids in Beimu by UPLC-ELSD.
10.19540/j.cnki.cjcmm.20191223.201
- Author:
Peng CHE
1
;
Jiu-Shi LIU
1
;
Yao-Dong QI
1
;
Ting-Yan QIANG
1
;
Yi-Chen SONG
1
;
Xue-Ping WEI
1
;
Hai-Tao LIU
1
;
Ben-Gang ZHANG
1
Author Information
1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Beimu;
UPLC-ELSD;
alkaloids;
quantitative analysis;
statistical analysis
- MeSH:
Alkaloids/isolation & purification*;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Fritillaria/chemistry*;
Phytochemicals/isolation & purification*;
Plant Roots/chemistry*
- From:
China Journal of Chinese Materia Medica
2020;45(6):1393-1398
- CountryChina
- Language:Chinese
-
Abstract:
An UPLC method was established for the direct determination of six major bioactive isosteroidal alkaloids, namely peimisine, imperialine, sipeimine-3-D-glucoside, verticinone, verticine and hupehenine from the bulbus of Fritillaria(Beimu), a commonly used antitussive traditional Chinese medicinal(TCM) herb. An Acquity UPLC~(TM) CSH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for all analysis. The investigated six compounds were all separated with gradient mobile phase consisting of 0.02% diethylamine-water-methanol at a flow rate of 0.3 mL·min~(-1). The temperature of sample manager was set at 20 ℃. Drift tube temperature was 45 ℃, and spray parameter was 40% with injection volume of 1 μL. Then, the further quality assessment of Beimu was carried out by cluster analysis(CA) and principal component analysis(PCA). The investigated all had good linearity(r≥0.998 9) over the tested ranges. The method is simple, accurate and reproducible, and can be used for determining the content of six major bioactive isosteroidal alkaloids.
