Isolation and identification of flavonoids from Spatholobi Caulis.
10.19540/j.cnki.cjcmm.20200202.201
- Author:
Xiao-Yan LIU
1
;
Wei XU
1
;
Xiu-Wei YANG
1
;
Peng ZHANG
2
;
Wei ZHAO
2
;
Yun GONG
2
;
Ni-Fu LIU
2
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences,Peking University Beijing 100191, China.
2. Zhuzhou Qianjin Pharmaceutical Co., Ltd. Zhuzhou 412000, China.
- Publication Type:Journal Article
- Keywords:
Spatholobi Caulis;
chemical compounds;
flavonoids;
isoflavones
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Fabaceae/chemistry*;
Flavonoids/isolation & purification*;
Phytochemicals/isolation & purification*
- From:
China Journal of Chinese Materia Medica
2020;45(6):1384-1392
- CountryChina
- Language:Chinese
-
Abstract:
The chemical compounds in water extract of Spatholobi Caulis were further studied. The compounds were systematically isolated and purified by using various separation and analysis techniques including silica gel, macroporous adsorptive resins and Sephadex LH-20 column chromatographies, as well as reversed phase high-performance liquid chromatography(RP-HPLC). Twenty-three flavonoids and one chromone were identified by the spectroscopic analysis techniques combining their physicochemical properties, they were identified as isoduartin(1), sativan(2), 8-O-methylretusin(3), 7-hydroxydihydroflavone(4), odoratin(5), butesuperin A(6), biochanin A(7), 3'-methoxydaidzein(8), 7-hydroxychromone(9), calycosin(10), naringenin(11), dihydrocajanin(12),(6 aR,11 aR)-maackiain(13), 2'-hydroxygenistein(14),(6 aR,11 aR)-medicarpin-3-O-glucopyranoside(15),(-)-epiafzelechin(16),(-)-catechin(17),(-)-epicatechin(18), 4',8-dimethoxy-7-O-β-D-glucopyranosylisoflavone(19), ononin(20),(-)-gallocatechin(21), rutin(22), daidzin(23) and sphaerobioside(24). Compounds 4, 6, 8, 9, 11, 12, 14-16, 19 and 22-24 were isolated from Spatholobi Caulis for the first time.
