Quality evaluation methods for standard decoction of Nelumbinis Folium.
10.19540/j.cnki.cjcmm.20191221.307
- Author:
Xue-Yuan WANG
1
;
Yun-Tao DAI
1
;
Ru-Na JIN
2
;
Qi-Shu JIAO
2
;
Shou-Gang SHI
3
;
Zheng-Jun HUANG
3
;
Shuo-Sheng ZHANG
4
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences Beijing 100700, China Shanxi University of Chinese Medicine Jinzhong 030619, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences Beijing 100700, China.
3. Sunflower Pharmaceutical Group Longzhong Co., Ltd. Xiangyang 441003, China.
4. Shanxi University of Chinese Medicine Jinzhong 030619, China.
- Publication Type:Journal Article
- Keywords:
Nelumbinis Folium;
extraction rate;
nuciferine;
quality evaluation;
quercetin-3-O-glucuronide;
standard decoction;
transfer rate
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Flowers/chemistry*;
Medicine, Chinese Traditional;
Nelumbo/chemistry*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2020;45(6):1363-1367
- CountryChina
- Language:Chinese
-
Abstract:
The quality evaluation method for standard decoction of Chinese herbal slices is the basis for the quality evaluation of granules and preparations of classical formula(decoction)of traditional Chinese medicine. This study aimed to establish a method for the determination of quercetin-3-O-glucuronic acid in Nelumbinis Folium(NF)and its standard decoction, so as to provide reference for the quality control of NF and its standard decoction. Fifteen batches of representative NF were collected to prepare standard decoction, and the parameters of dry extract rate, transfer rate of index component, and pH value were calculated. HPLC was used to establish the content determination method for quercetin-3-O-glucuronic acid in NF and its standard decoction. The concentration range of quercetin-3-O-glucuronic acid in the standard decoction of NF was 1.09-3.06 g·L~(-1), while the concentration range of nuciferine was 0.01-0.17 g·L~(-1). The average extraction rate of NF standard decoction was(14.4±2.6)%, the average transfer rate of quercetin-3-O-glucuronic acid was(70.7±18.6)%, and the average transfer rate of nuciferine was(9.6±5.4)%. Compared with Nuciferine, quercetin-3-O-glucuronic acid had a high content and stable transfer rate in standard decoction, and was recommended to be the quality control marker for NF and its standard decoction. This paper establishes a quality evaluation method for NF standard decoction, and can provide reference for the quality control of all preparations derived from NF and its decoction.
