Antipyretic effect of active components of Mahuang Decoction and its correlation with pharmacokinetics in febrile rats.
10.19540/j.cnki.cjcmm.20190524.501
- Author:
Guang-Wei CHEN
1
;
Yan-Fang TIAN
1
;
Hai-Tong WAN
1
;
Jian-Zhen CHEN
1
;
Yu HE
1
Author Information
1. Zhejiang Chinese Medical University Hangzhou 310053, China.
- Publication Type:Journal Article
- Keywords:
Mahuang Decoction;
PK-PD correlation;
active components;
antipyretic effect;
febrile model rats
- MeSH:
Animals;
Antipyretics/therapeutic use*;
Drugs, Chinese Herbal/therapeutic use*;
Ephedra sinica/chemistry*;
Fever/drug therapy*;
Interleukin-1beta/blood*;
Interleukin-6/blood*;
Medicine, Chinese Traditional;
Rats;
Tumor Necrosis Factor-alpha/blood*
- From:
China Journal of Chinese Materia Medica
2020;45(3):655-663
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the antipyretic effect of active components of Mahuang Decoction in febrile rats, and explore its correlation with pharmacokinetics at different time points. The feverished rat models were induced by dry yeast, and intragastrically administered with the effective components of Mahuang Decoction with different orthogonal compatibility ratios. At different time points after administration, body temperature was measured; blood was taken from orbital vena plexus, and the contents of interleukin-6(IL-6), interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) in rat serum were determined with the kits. Combined with the pharmacokinetic data of the seven effective components in Mahuang Decoction, PK-PD(pharmacokinetics-pharmacodynamics) data fitting was conducted by using the analysis method of non-atrioventricular model, and then the pharmacodynamic parameters were calculated to determine the optimal binding model. The results showed that the effective components of Mahuang Decoction inhibited the release of heat-causing factors IL-6, IL-1β and TNF-α, and reduced the increase of body temperature. There was a significant lag between drug effect and blood drug concentration, which was consistent with Sigmoid-E_(max) model. The model fitting value showed a good correlation with mea-sured data, which could be used to evaluate and predict the correlation between PK and PD in Mahuang Decoction, and further applied to the multiple-indicator and multiple-effect study of PK-PD in other compound traditional Chinese medicines.
