UPLC characteristic fingerprint and chemical pattern recognition of Angong Niuhuang Pills.
10.19540/j.cnki.cjcmm.20191217.305
- Author:
Yu-Shuang CHAI
1
;
Hu-Hu ZENG
2
;
Yuan-Feng HE
1
;
Xiao SHANG
2
;
Ting WAN
1
;
Zhen YIN
1
;
Chun-Lin FAN
2
;
Wen-Cai YE
2
Author Information
1. Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd. Guangzhou 510530, China.
2. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University Guangzhou 510632, China.
- Publication Type:Journal Article
- Keywords:
Angong Niuhuang Pills;
chemical pattern recognition;
fingerprint;
ultra performance liquid chromatography
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(3):565-571
- CountryChina
- Language:Chinese
-
Abstract:
To establish the UPLC fingerprint of Zhongyi Angong Niuhuang Pills, in order to evaluate its quality by chemical pattern recognition. The method was developed on a column of Poroshell 120 EC-C_(18), with methanol-0.1% formic acid solution as the mobile phase for gradient elution at a flow rate of 0.4 mL·min~(-1). The column temperature was 30 ℃,and the detective wavelength was 254 nm. The similarity of 24 batches of Angong Niuhuang Pills was compared by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004 A). Hydrophobic cluster analysis,principal components analysis and partial least squares discriminant analysis were conducted by using SIMCA 13.0 software to investigate different components among these products. The UPLC characteristic fingerprint was established in this study. And 17 common peaks were identified by standard reference and UPLC-MS. The similarity of 24 batches samples were above 0.980,which can be classified into three categories for pattern recognition. Baicalin,berberine,jatrorrhizine,wogonin and wogonoside were identified as the main markers that cause differences of various batches. The method is simple,rapid,accurate and reproducible,and can provide scientific basis for improving the quality standard of Zhongyi Angong Niuhuang Pills.
