Migration of phenolic active ingredients in Danshen Dizhuye during ultrafiltration of hollow fiber membrane.
10.19540/j.cnki.cjcmm.20191217.302
- Author:
Ya-Ping CHEN
1
;
Gui-You WANG
2
;
Liang FENG
1
;
Cheng-Liang QI
2
;
Cong-Ping CHEN
2
;
Bing YANG
1
;
Xin-Hong SHI
1
;
Xiao-Bin JIA
1
Author Information
1. School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China.
2. Anhui Tianyang Pharmaceutical Co., Ltd. Tianchang 239300, China.
- Publication Type:Journal Article
- Keywords:
Danshen Dizhuye;
membrane ultrafiltration;
migration regularity;
salvianolic acid
- MeSH:
Alkenes/chemistry*;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Polyphenols/chemistry*;
Salvia miltiorrhiza/chemistry*;
Ultrafiltration
- From:
China Journal of Chinese Materia Medica
2020;45(3):548-554
- CountryChina
- Language:Chinese
-
Abstract:
Study the suitability of organic film for salvianolic acid in the ultrafiltration process of Danshen Dizhuye. UPLC was used to analyze the migration of nine phenolic active ingredients in Danshen Dizhuye during ultrafiltration of PES hollow fiber membrane and PS hollow fiber membrane. The structural composition of multi-components was analyzed by three different batches of Danshen Dizhuye before and after ultrafiltration of the two membranes. The results showed that 9 phenolic active ingredients in Danshen Dizhuye did not change significantly after ultrafiltration through PES membrane. However, after ultrafiltration through PS membrane, the content of sodium danshensu, protocatechualdehyde, caffeic acid, 3-hydroxy-4-methoxycinnamic acid and rosmarinic acid in Danshen Dizhuye did not change significantly, while salvianolic acid D, salvianolic acid B and lithospermic acid decreased by about 20%, and the content of salvianolic acid A decreased significantly. The final content in equilibrium was only about 20% of the original solution. Therefore, an in-depth study on the migration particularity of salvianolic acid A in ultrafiltration membrane was the focuse. The results showed that the loss of salvianolic acid A was caused by both membranes during ultrafiltration, and salvianolic acid A was lost more in PS membrane. When the membrane was washed and regenerated, it was found that salvianolic acid A was detected in the ethanol washing solution, but not in the washing liquid, indicating that the loss of salvianolic acid A during the ultrafiltration was mainly adsorptive action. The results suggested that the migration of phenolic active ingredients in Danshen Dizhuye during the membrane ultrafiltration process did not completely follow the molecular weight passing rule of the membrane pore size. At the same time, it may be affected by factors, such as the structure of the membrane material, and the interaction between the membrane structure and the structure of components, and exhibit different migration behaviors during the ultrafiltration of the membrane.
