HPLC specific chromatogram of Vernonia anthelmintica and determination of six components.
10.19540/j.cnki.cjcmm.20191111.201
- Author:
Zong-Yang LIU
1
;
Ke ZAN
2
;
La Maiti Ai-Li SHA
3
;
Xia WU
1
;
Li-Nong GUO
2
;
Shuang-Cheng MA
2
;
Jian ZHENG
2
;
Xiao-Qing CHEN
1
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
2. National Institutes for Food and Drug Control Beijing 100050, China.
3. Xinjiang Uyghur Autonomous Region Institute for Food and Drug Control Urumqi 830004, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Uyghur medicine;
Vernonia anthelmintica;
multi-components determination;
specific chromatogram
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Phytochemicals/analysis*;
Quality Control;
Vernonia/chemistry*
- From:
China Journal of Chinese Materia Medica
2020;45(4):910-915
- CountryChina
- Language:Chinese
-
Abstract:
This work aims to establish an HPLC specific chromatogram and determine six components of Vernonia anthelmintica with chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, scutellarein and luteolin as index components. HPLC analysis was performed on a Waters Xbridge C_(18) column(4.6 mm×250 mm, 5 μm) with gradient elution of acetonitrile-0.05% trifluoroacetic acid solution at a flow rate of 1.0 mL·min~(-1). The detection wave length was 360 nm and the column temperature was 30 ℃. Chemometrics software Chempattern was employed to analyze the data. HPLC specific chromatogram of V. anthelmintica was established and six characteristic peaks were marked. Six characteristic peaks were simultaneously determined by HPLC within 50 min. The contents of the six components in 13 batch samples of V. anthelmintica were 0.14%-0.68%, 0.44%-0.74%, 0.63%-1.01%, 0.14%-0.71%, 0.15%-0.26% and 0.010%-0.030%, respectively. The HPLC specific chromatogram of V. anthelmintica, together with determination of six components showed strong specificity, and it can be used for the quality control of the crude drug.
